麦麸酶解产物对糖尿病大鼠肝脏和睾丸氧化损伤的保护作用

目的:观察麦麸酶解产物(EHWB)对糖尿病大鼠肝脏和睾丸的抗氧化作用。方法:用四氧嘧啶制备SD大鼠糖尿病模型,以EHWB 19.36mg/kg bw.d灌胃,维生素C(VC)10.42mg/kg bw.d灌胃作对照,测定各实验组大鼠血糖,肝脏和睾丸总抗氧化能力(T-AOC)及超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和黄嘌呤氧化酶(XOD)活性及丙二醛(MDA)含量。结果:与糖尿病组相比,EHWB治疗组大鼠血糖明显降低(P<0.01=,肝脏和睾丸T-AOC能力增强(P<0.01),GSH-Px和SOD活力增强(P<0.05),XOD活性和MDA含量降低(P<0.05)。EHWB治疗组大鼠肝脏和睾丸T-AOC能力明显高于VC组(P<0.01),肝脏XOD活性低于VC组(P<0.01)。结论:EHWB具有降血糖和提高糖尿病大鼠组织抗氧化能力,其效果强于传统抗氧化剂VC。

THE PROTECTIVE EFFECTS OF ENZYMES HYDROLYZED WHEAT BRAN ON THE OXIDATIVE DAMAGE OF LIVER AND TESTIS IN DIABETIC RATS

Objective: To observe the antioxidative function of enzymes-hydrolyzed wheat bran(EHWB) on liver and testis in diabetic rats.Method: Diabetes mellitus was induced by alloxan injection.EHWB was given intragastrically(ig),and vitamin C(VC) was given as control group.The level of blood glucose,the total antioxidative capacity(T-AOC),the activity of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),and xanthine oxidase(XOD),and the content of malondiadehyde(MDA) were detected respectively in liver and testis.Results: EHWB significantly decreased the level of blood glucose and increased the level of T-AOC,as well as the activity of GSH-Px and SOD,but decreased the activity of XOD and the content of MDA in liver and testis of diabetes rats.The level of T-AOC in liver and testis of rats fed EHWB was higher and the activity of XOD in liver was lower than those in rats fat VC.Conclusion: EHWB can effectively decrease the level of blood glucose and increase antioxidative effects of diabetic rats,and the effects are stronger than VC.