Blockade of angiotensin II AT1 receptors inhibits pressor action of centrally administered interleukin-1β in Sprague Dawley rats |
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Authors: | Marcin Ufnal Tymoteusz era Ewa Szczepaska-Sadowska |
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Institution: | Department of Experimental and Clinical Physiology, Medical University of Warsaw, Krakowskie Przedmiescie 26/28, 00-927 Warsaw, Poland |
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Abstract: | The aim of the present study was to evaluate the influence of intraventricular administration of recombinant rat interleukin-1β (IL-1β) on regulation of resting blood pressure and heart rate and to test the hypothesis that the brain angiotensinergic system is involved in regulation of hemodynamic parameters by centrally applied IL-1β. The experiments were performed on Sprague Dawley rats, assigned to three series of experiments. In series 1 (control, n = 6), mean arterial pressure (MAP) and heart rate (HR) were recorded for 15 min under baseline conditions. This was followed by infusion of saline (0.9% sterile NaCl 5 μL/h) into the left cerebral ventricle (LCV). Measurements were continued during the next 60 min. In series 2 (n = 6) and 3 (n = 6) the experimental design was similar, except that in series 2 the animals were LCV infused with saline containing IL-1β (100 ng/h) and in series 3 with saline containing IL-1β (100 ng/h) and angiotensin type 1 (AT1) receptors antagonist (Losartan, 10 μg/h). LCV infusion of saline alone did not influence MAP and HR while administration of IL-1β elicited significant increase in MAP, but not in HR. The pressor effect was absent during combined infusion of IL-1β and Losartan. Results of the study provide evidence that centrally administered IL-1β exerts pressor effect, and reveal that this effect is mediated by stimulation of the brain angiotensin system and requires activation of AT1 receptors. |
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Keywords: | Interleukin-1β Angiotensinergic system Central cardiovascular regulation Rat |
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