石蜡半薄切片制作透射电镜的方法

[目的]介绍石蜡半薄切片转制透射电镜的制样方法。[方法]对石蜡半薄切片进行脱蜡、水化、锇酸固定、缓冲液漂洗、梯度酒精脱水、环氧树脂包埋、超薄切片机切片、透射电镜下观察。[结果]组织细胞的超微结构保存完好,足以满足病理诊断之用。[结论]用石蜡半薄切片转制电镜比用石蜡组织块转制电镜方法简单,快速易行。

Sample preparation methods of TEM using semi-thin paraffin sections

[Objective] To introduce sample preparation methods of TEM using semi-thin paraffin sections.[Methods] The semi-thin paraffin section was processed with dewaxing,hydration,osmium tetroxide fixed,buffer rinsing,gradient alcohol dehydration,epoxy resin embedded and ultra-thin slice.Then the sections were observed under the Transmission Electron Microscopy.[Results] The ultra-structure of histo-cyte remained well.It was sufficient to meet the requirement of pathological diagnosis.[Conclusion] Compared with par...