| 人转铁蛋白-阿霉素结合物的制备及其体外细胞毒作用 |
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| 引用本文： | 林溪,黄秀旺.人转铁蛋白-阿霉素结合物的制备及其体外细胞毒作用[J].肿瘤,2000,20(4):263-265. |
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| 作者姓名： | 林溪 黄秀旺 |
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| 作者单位： | 福建医科大学临床药理研究所!福州350004 |
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| 摘 要： | 目的 介绍人转铁蛋白－阿霉素结合物的制备及细胞毒性。方法 采用氧化葡聚糖（ＤＥＸ）Ｔ－４０作为中介体，联结人转铁蛋白（ＨＴＦ）与阿霉素（ＡＤＲ）制备人转铁蛋白－阿霉素交联物９ＨＴＦ－ＤＥＸ－ＡＤＲ）。ＨＴＦ与ＡＤＲ的克分子比为１：４０￣４５。以ＭＴＴ法测其细胞毒性。结果 交联物对肿瘤细胞Ｋ５６２１ｈ处理的ＩＣ５０中游离ＡＤＲ的４．２倍，对正常人单核细胞４８ｈ处理的杀伤只有游离ＡＤＲ的１／９。结论
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| 关 键 词： | 转铁蛋白 阿霉素 肿瘤细胞 体外细胞毒作用 |
PREPARATION OF HUMAN TRANSFERRIN-ADRIAMYCIN CONJUGATE AND CYTOTOXIC EFFECT OF THE CONJUGATES IN VITRO |
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| LIN Xi,HUANG Xiu wang Institute of Clinical Pharmacology,Fujian Medical University,Fu zhou China.PREPARATION OF HUMAN TRANSFERRIN-ADRIAMYCIN CONJUGATE AND CYTOTOXIC EFFECT OF THE CONJUGATES IN VITRO[J].Tumor,2000,20(4):263-265. |
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| Authors: | LIN Xi HUANG Xiu wang Institute of Clinical Pharmacology Fujian Medical University Fu zhou China |
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| Institution: | LIN Xi,HUANG Xiu wang Institute of Clinical Pharmacology,Fujian Medical University,Fu zhou 350004 China |
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| Abstract: | Objective Preparation of human transferrin adriamycin conjugate and its cytotoxicity was described. Methods Human transferrin (HTF) was conjugatd with adriamycin (ADR), via oxidative dextran T 40 as linker, to construct HTF DEX ADR. The molarratio of HTF to drug was to 40 45. The cytotoxicity of HTF DEX ADR to K562 cells was determined by MTT method.Results The cytotoxicity of HTF DEX ADR to K562 cells was 4 2 times than that of free ADR, while to normal peripheral blood mononuclear cells was only 1/9 that of free ADR. Conclusion It suggests that the conjugate shows a selective cytotoxicity to K562 cells.$$$$ |
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| Keywords: | Transferrin Adriamycin Tumor cells cultured |
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