| 应用差别PCR检测石蜡包埋乳腺癌组织癌基因扩增时固定剂影响的研究 |
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| 引用本文： | 陈森清,马国建.应用差别PCR检测石蜡包埋乳腺癌组织癌基因扩增时固定剂影响的研究[J].癌变．畸变．突变,1996,8(2):72-74. |
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| 作者姓名： | 陈森清 马国建 |
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| 作者单位： | 江苏省肿瘤防治研究所 |
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| 摘 要： | 应用差别ＰＣＲ检测石蜡包埋乳腺癌组织癌基因扩增时固定剂影响的研究陈森清，马国建，吴建中，薛开先江苏省肿瘤防治研究所南京２１０００９ＥＦＦＥＣＴＳＯＦＦＩＸＡＴＩＶＥＩＮＰＡＲＡＦＦＩＮ－ＥＭＢＥＤＥＤＢＲＥＡＳＴＣＡＮＣＥＲＴＩＳＳＵＥＯＮＤＥＴＥＣ...
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| 关 键 词： | 乳腺癌 癌基因 PCR 检测 固定剂 |
EFFECTS OFFIXATIVEINPARAFFIN- EMBEDEDBREASTCANCERTISSUEONDETECTION PFONCOGENEAMPLIFICA- TIONUSINGDIFFERENTIALPCR |
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| Chen Scnqing; Ma Guojian; Wu Jianzhong; Xue Kaixian.EFFECTS OFFIXATIVEINPARAFFIN- EMBEDEDBREASTCANCERTISSUEONDETECTION PFONCOGENEAMPLIFICA- TIONUSINGDIFFERENTIALPCR[J].Carcinogenesis,Teratogenesis and Mutagenesis,1996,8(2):72-74. |
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| Authors: | Chen Scnqing; Ma Guojian; Wu Jianzhong; Xue Kaixian |
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| Abstract: | Effects of fixative and DNA extraction process from paraffin- embeded breastcancer tissue on detecting oncogene amplification using differential PCR(D-PCR)werestudied.The results showed that D-PCR would be satisfied from breastcancer tissue fixedwith 10%formalin or absulute alcohol,while D- PCR was unstable when fixed with 20%formalin.Further analysis implicated that high concentration of formalin could induce DNAfragments. |
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| Keywords: | differential PCR fixative oncogene amplification |
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