| 三氧化二砷诱导HL-60细胞凋亡的实验研究 |
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| 引用本文： | 王展翔,许勇钢,廖军鲜,麻柔,周霭祥.三氧化二砷诱导HL-60细胞凋亡的实验研究[J].中国中西医结合杂志,2000,20(7):536-538. |
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| 作者姓名： | 王展翔 许勇钢 廖军鲜 麻柔 周霭祥 |
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| 作者单位： | |
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| 摘 要： | 目的：探讨三氧化二砷（Ａｓ２Ｏ３）治疗白血病的疗效机理。该当以ＨＬ－６０细胞株为体外模型，终浓度为０．２０μｍｏｌ／ＬＡｓ２Ｏ３作用２４、４８ｈ后，用ＰＩ／ＡｎｎｅｘｉｎＶ－ＦＩＴＣ双标记，流式细胞术观察Ａｓ２Ｏ３对ＨＬ－６０细胞凋亡及其对细胞株端粒酶活性的影响。结果：Ａｓ２Ｏ３作用于ＨＬ－６０细胞后ＰＩ阴性／ＡｎｎｅｘｉｎＶ－ＦＩＴＣ细胞性细胞增多，提示细胞发生凋亡，ＤＮＡ周期分析显示主要作用在
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| 关 键 词： | 三氧化二砷 HL－60 细胞凋亡 端粒酶 白血病 |
Experimental Study on Apoptosis of HL-60 Cell Induced by Arsenic Trioxide |
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| Authors: | WANG Zhanxiang XU Yonggang LIAO Junxian |
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| Institution: | Department of Hematology, Xiyuan Hospital, China Academy of Traditional Chinese Medicine, Bejing (10091). |
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| Abstract: | To explore the mechanism of arsenic (arsenic trioxide, As_2O_3) in treating leukemia. Methods: The in vitro effect of arsenic trioxide on HL-60 model double-labelled with PI/AnnexinV-FITC was studied with flow cytometry. Function on telomerase activity was also observed. Results: Alter the effect of arsenic on HL60 cell line, PI negative/ AnnexinV- FITC positive cells increased, it mainly acts at t he G2 - M phase that suggests the apoptosis takes place,. mild inhibition on telomerase was also found 48 hours later. Conclusion: Apoptosis induced by arsenic trioxide was the main mechanism in killing or inhibiting leukemic cells. |
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| Keywords: | arsenic trioxide cell line HL-60 apoptosis telomerase |
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