巨噬细胞阳性蛋白-3α、半胱氨酸蛋白酶抑制剂A及前梯度蛋白-2在鼻咽癌患者中的表达及预后判断

目的 研究巨噬细胞炎性蛋白-3α(MIP-3α)、半胱氨酸蛋白酶抑制剂A(cystatin A)及前梯度蛋白-2(AGR-2)在鼻咽癌患者中的表达情况及用于判断预后的价值。方法 选择2012年1月~2013年1月于陕西省核工业二一五医院就诊的鼻咽癌患者90例,采用免疫组织化学方法检测患者癌组织及癌旁组织MIP-3α、cystatin A及AGR-2的表达。利用Kplan-Merier曲线和Cox比例风险模型分析MIP-3α、cystatin A及AGR-2评估鼻咽癌预后的价值。结果 癌组织MIP-3α阳性患者49例(54.4%),cystatin A阳性59例(65.6%),AGR-2阳性53例(58.9%),分别显著多于癌旁组织MIP-3α阳性22例(24.4%,χ²=23.320, P<0.001),cystatin A 25例(27.8%,χ²=25.804,P<0.001),AGR-2 28例(31.1%,χ²=14.029,P<0.001)。MIP-3α、AGR-2阳性患者的总生存率(OS)、无远端转移生存率(DMFS)差于MIP-3α阴性患者(P<0.005),cystatin A阳性患者的OS、局部无复发生存率(LRFS)、DMFS均差于cystatin A阴性患者(P<0.005)。Cox风险比例发现,MIP-3α、cystatin A及AGR-2可以作为鼻咽癌患者预后的有效预测因子。结论 MIP-3α、cystatin A及AGR-2在鼻咽癌患者癌组织中高表达,与鼻咽癌患者预后相关,可以作为一种标记物判断患者预后。

Expression and prognosis of macrophage positive protein-3α and cysteine proteinase inhibitor A and anterior gradient-2 in nasopharyngeal carcinoma patients

Objective To investigate the expression of macrophage inflammatory protein-3α (MIP-3α), cysteine protease inhibitor A (cystatin A) and anterior gradient-2 protein-2 (AGR-2) in patients with nasopharyngeal carcinoma and its prognostic value.Methods Ninety patients with nasopharyngeal carcinoma who were treated at the 215 Hospital of Nuclear Industry of Shaanxi Province from January 2012 to January 2013 were enrolled. Immunohistochemical method was used to detect the expression of MIP-3α, cystatin A and AGR-2 in cancer tissues and adjacent tissues. The prognostic value of MIP-3α, cystatin A and AGR-2 was evaluated by Kplan-Merier curve and Cox proportional hazard model.Results The numbers of patients with positive MIP-3α(45,54.5%), positive cystatin A(59,65.6%), and positive AGR-2(53,58.9%) in the cancer tissues were significantly more than those in the adjacent tissues with positive MIP-3α(22,24.4%; χ²=23.320, P<0.001), positive cystatin A (25, 27.8%; χ²=25.804,P<0.001), positive AGR-2 (28, 31.1%; χ²=14.029,P<0.001). The overall survival (OS), distant metastasis survival (DMFS) of positive MIP-3α, AGR-2 patients were worse than those of MIP-3α negative (P<0.005). The local recurrence free survival (LRFS),OS,DMFS of patients with positive cystatin A was worse than that of patients with negative cystatin A (P<0.005). The Cox risk ratio showed that MIP-3α, cystatin A and AGR-2 can be used as effective predictors of prognosis in nasopharyngeal carcinoma patients.Conclusions MIP-3α, cystatin A and AGR-2 are highly expressed in patients with nasopharyngeal carcinoma and are associated with the prognosis. These predicts could be used as markers for evaluating the prognosis of nasopharyngeal carcinoma patients.