五虎凝胶贴膏质量标准研究

目的 建立五虎凝胶贴膏的质量标准.方法 采用薄层色谱法(TLC)对处方中白芷、当归、防风、红花、制天南星进行定性鉴别;采用HPLC同时测定处方中羟基红花黄色素A、阿魏酸、升麻素苷、5-O-甲基维斯阿米醇苷、欧前胡素、异欧前胡素的含量,色谱柱为Agilent Eclipse Plus C18(250 mm×4.6 mm,...

Study on Quality Standard for Wuhu Gel Paste

OBJECTIVE To establish the quality standard for Wuhu Gel Paste. METHODS TLC was adopted to identify Angelica Dahurica, Angelicae Sinensis Radix, Saposhnikovia Divaricate, Carthamii Flos, Dried Ginger. HPLC was used to determine hydroxysafflor yellow A, ferulic acid, prim-O-glucosylcimifugin, 4-O-β-D-glucosyl-5-O-methylvisamminol, imperation and isoimperation. The analysis was performed on a 28℃ thermostatic Agilent Eclipse C₁₈ column(250 mm×4.6 mm, 5 μm), with the mobile phase comprising of methanol-0.025% phosphoric acid flowing at 1.0 mL·min^-1 in a gradient elution manner, and the detection wavelengths were set at 403, 300, 254 nm. RESULTS The medicinal flavours had clear thin spots, good separation and no negative interference. Six constituents showed good linear relationships within their own ranges(r ≥ 0.999 5), while the average recoveries were 96.30%-100.56% with the RSDs of 1.06%-1.65%. CONCLUSION The TLC method in this experiment has strong specificity and good separating degree. The HPLC method has high accuracy and repeatability. The established methods can be used to control quality of Wuhu Gel Paste.