氯普鲁卡因通过调控长链非编码 RNA TTN?AS1表达对胶质瘤细胞增殖和转移的影响

目的 探讨氯普鲁卡因(CP)对胶质瘤细胞增殖和转移的影响及分子机制.方法 用浓度为1 mmol/L、3 mmol/L、9 mmol/L的CP培养U251细胞作为CP低、中、高浓度组;未经任何处理的细胞作为对照组;此外将U251细胞分为si-NC组、si-TTN-AS1组、CP+pcDNA组、CP+pcDNA-TTN-AS1组.噻唑兰(MTT)检测细胞增殖抑制率;Transwell检测细胞迁移和侵袭;实时荧光定量PCR(RT-qPCR)检测lncRNA TTN-AS1表达水平.结果 CP处理胶质瘤U251细胞后,细胞增殖抑制率升高,细胞迁移侵袭数以及TTN-AS1表达水平降低(P<0.05).抑制TTN-AS1表达可抑制胶质瘤细胞增殖、迁移和侵袭.TTN-AS1过表达逆转了CP对U251细胞的作用.结论 CP通过下调lncRNA TTN-AS1表达抑制胶质瘤细胞增殖和转移.

Effects of chloroprocaine on the proliferation and metastasis of glioma cells by regulating the expression of lncRNA TTN-AS1

Objective To investigate the effect of chloroprocaine (CP) on the proliferation and metastasis of glioma cells and its molecular mechanism.Methods U251 cells were cultured with chloroprocaine at a concentration of 1 mmol/L, 3 mmol/L, and 9 mmol/Las the low, medium, and high concentration groups of chloroprocaine; cells without any treatment were used as the control group. In addition, U251 cells were divided into si-NC group, si-TTN-AS1 group, CP+pcDNA group, CP+pcDNA-TTN-AS1 group. Thiazole blue(MTT) was used to detect the inhibition rate of cell proliferation; Transwell was used to detect cell migration and invasion; real-time quantitative PCR (RT-qPCR) was used to detect the expression of lncRNA TTN-AS1.Results After chloroprocaine treatment of glioma U251 cells, the cell proliferation inhibition rate was significantly increased, and the number of cell migration and invasion, TTNAS1 expression were reduced (P<0.05). Inhibition of TTN-AS1 expression can inhibit glioma cell proliferation, migration and invasion. TTN-AS1 overexpression reversed the effect of chloroprocaine on U251 cells.Conclusion Chloroprocaine inhibits the proliferation and metastasis of glioma cells by down-regulating the expression of lncRNA TTN-AS1.