Quantitative functional analysis of protein complexes on surfaces |
| |
| Authors: | Hye Jin Lee Yuling Yan Gerard Marriott Robert M. Corn |
| |
| Affiliation: | Department of Physiology, University of Wisconsin–Madison, WI, USA;Department of Mechanical Engineering, University of Hawai'i Manoa, HI, USA;Department of Chemistry, University of California–Irvine, CA, USA |
| |
| Abstract: | A major challenge in cell and molecular physiology research is to understand the mechanisms of biological processes in terms of the interactions, activities and regulation of the underlying proteins. Functional and mechanistic analyses of the large number of proteins that participate in the regulation of cellular processes will require new approaches and techniques for high throughput and multiplexed functional analyses of protein interactions, protein conformational dynamics and protein activity. In this review we focus on the development and application of proteomics and associated technologies for quantitative functional analysis of proteins and their complexes that include: (1) the application of surface plasmon resonance (SPR) imaging for multiplexed, label-free analyses of protein interactions, binding constants for biomolecular interactions and protein activities; and (2) high content analysis of protein motions within functional multiprotein complexes. |
| |
| Keywords: | |
|
|
