肥大细胞类胰蛋白酶在病理性瘢痕中的表达研究

目的 研究肥大细胞类胰蛋白酶(mast cell tryptase,MCT)在病理性瘢痕中的表达及分布情况,探讨MCT基因在瘢痕疙瘩、增生性瘢痕及正常皮肤中是否存在差别.方法 采集未经治疗的瘢痕疙瘩、增生性瘢痕及正常皮肤各20例,应用免疫荧光组化对MCT的表达进行定位,应用实时荧光相对定量PCR进行mRNA基因水平的相对定量分析.结果 MCT主要集中在瘢痕组织的胶原纤维柬之间,以瘢痕组织浅层分布较多;实时荧光PCR相对定量结果显示MCT基因在瘢痕疙瘩中表达高于增生性瘢痕和皮肤(P<0.01),瘢痕疙瘩中MCT基因表达量约为增生性瘢痕的2.5倍,皮肤的5.4倍.结论 MCT在瘢痕的形成中可能起一定的作用.

Expression of mast cell tryptase in Scar

Objective To investigate the expression and distribution of mast cell tryptase (MCT) in scar, and to discuss the different MCT gene expression in keloid, hypertrophic scar and normal skin. Methods 20 samples of keloid, 20 samples of hypertrophic scar and 20 samples of normal skin were collected. The distribution of MCT was investigated by immunofluorescence histochemistry, and the MCT mRNA expression was detected by Relative Quantification real-time fluorescent PCR. Results MCT gene was mainly located in the collagen fiber bundles of the scar, especially in the superficial layer of scar. MCT mRNA expression was significantly higher in keloid than that in hypertrophic scar and normal skin (P < 0. 01) . Averagely, the MCT gene expression in keloid was 2. 5 times and S. 4 times of that in hypertrophic scar and normal skin. Conclusions MCT gene may play a role in the pathogenesis of scar.