甾体激素和血管活性物质ET/NO对培养的人子宫动脉平滑肌细胞增值的影响

利用体外培养系统，通过3H－TdR掺入和MTT法观察了甾体激素和血管活性物质内皮素（ET）与一氧化氮（NO）在培养的人子宫动脉平滑肌细胞（HUASMC）增殖中的作用。结果：雌激素对HUASMC的DNA合成和细胞增殖无影响。孕激素和ET－1可促进HUASMC DNA合成和细胞增殖，其作用呈剂量依赖性。孕激素可明显增强HUASMC对ET－1的增殖反应，NO供体硝普钠（SNP）抑制 HUASMC DNA合成和细胞增殖，其作用强度与浓度呈正相关。雌激素可增强SNP对HUASMC的抑制作用，孕激素无此作用。结果提示 ET－1和 NO在子宫血流调节和内膜血管重建中具有重要作用并受卵巢激素调节。

The Effect of Endothelin Nitric Oxide and Sex Steroid Hormones on Proliferation of Human Uterine Artery Smooth Muscle Cells in Culture

The effect of endothelin (ET)、nitric oxide(NO) and ovarian hormones on proliferation of human uterine artery smooth muscle cell (HUASMC) in culture was studied using 3H-TdR in corporation and MTT method. Estradiol had no significant effect on HUASMC proliferation. ET-1and progesterone could promote DNA synthesis and cell proliferation of HUASMC in dose dependent manner (P<0. 05,P<0. 01),this effect could be intensed by combined ET-1 and progesterone (P<0. 01), but sodium nitroprusside (SNP) which could provide NO, antagonized the proliferative effect(p<0. 01) and significantly inhibited DNA synthesis and cell proliferation of HUASMC (P<0. 01), its effect could be intensed by estradiol but not by progesterone (P<0.01). It is concluded that ET-1 and NO might take part in the regulation of uterine blood flow and reconstruction of endometrium arteries and their effect is regulated by ovarian hormones.