| 河北省食品中大肠杆菌O157：H7分布及毒力研究 |
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| 引用本文： | 张淑红,申志新,关文英,侯凤伶,王英豪.河北省食品中大肠杆菌O157：H7分布及毒力研究[J].中国卫生检验杂志,2007,17(1):120-122. |
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| 作者姓名： | 张淑红 申志新 关文英 侯凤伶 王英豪 |
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| 作者单位： | 河北省疾病预防控制中心 河北保定071000 |
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| 摘 要： | 目的:掌握河北省食品中大肠杆菌O157:H7分布状况及菌株的毒力研究。方法:运用免疫磁珠富集进行分离培养,并用PCR进行毒力基因分析。结果:共采集食品530份,检出O157:H7大肠杆菌5株,总检出率为0.9%。其中,以生牛、羊肉检出率较高,分别为6.7%和4.4%。5株O157:H7菌株中,4株带有SLT2、eae和H ly 3种毒力基因,1株带有SLT1、SLT2、eae和H ly 4种毒力基因。结论:掌握食品中O157:H7分布及生物学特性对预防食源性疾病暴发有重要意义。
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| 关 键 词： | 食品 大肠杆菌O157:H7 分布 毒力基因 |
| 文章编号： | 1004-8685（2007）01-0120-03 |
| 收稿时间： | 2006-08-07 |
| 修稿时间： | 2006年8月7日 |
Escherichia coli O157:H7 among food and toxic gene of strain in Hebei Province |
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| Zhang Shu-hong , Shen Zhi-xin , Guan Wen- ying , Hou Feng-ling , Wang Ying-hao.Escherichia coli O157:H7 among food and toxic gene of strain in Hebei Province[J].Chinses Journal of Health Laboratory Technology,2007,17(1):120-122. |
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| Authors: | Zhang Shu-hong Shen Zhi-xin Guan Wen- ying Hou Feng-ling Wang Ying-hao |
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| Institution: | Hebei Center for Disease Control and Prevention, Baoding 071000, China |
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| Abstract: | Objective:To detect the Escherichia coli O157:H7 positive rate of food and the toxic gene of the strain in Hebei Province.WTHZ]Methods:Escherichia coli O157:H7 was isolated with immune magnetic essay from foods and PCR was performed to detect the toxic gene of the strains.Results:Altogether 5 strains of O157:H7 were separated from 530 feces of different foods and the overall positive rate was 0.9%.The positive rates of cattle and sheep was high and it was 6.7% and 4.4% respectively.For 5 strains,SLT1,SLT2,eaeA and lily toxic genes were detected,4 strains carried SLT2,eae and Hly genes,1 strain carried SLT1,SLT2,eae and Hly genes.Conclusion:To enhance the surveillance of O157:H7 in food should be very important for the prevention and control of the foodborne disease outbreaks. |
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| Keywords: | Food Escherichia coli O157:H7 Host animal Toxic gene |
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