养心通脉片对动脉粥样硬化白兔VEGF的表达和新生血管数的影响

目的观察养心通脉片对动脉粥样硬化（AS）白兔血管内皮生长因子（VEGF）的表达和新生血管数的影响和机制.方法利用高脂饲料喂养白兔造模,正常组和模型组用双蒸水灌胃,养心通脉片组用养心通脉液灌胃.1个月后,取出白兔主动脉,运用免疫组化法来观察VEGF和新生血管数.结果模型组和养心通脉片组主动脉斑块都可见棕黄色阳性表达,模型组表达较为强烈,两者比较有统计学意义（P〈0.05）.两组主动脉斑块都可见大于10 μm的颗粒,养心通脉片组颗粒较少,与模型组比较有统计学意义（P〈0.01）.结论养心通脉片在动脉粥样斑块形成后,通过影响VEGF和新生血管的表达来有效地逆转或消退AS斑块.

The Effect of Yangxintongmai Tablet on the Expression of VEGF in Rabbits with Atherosclerosis

Objective To investgate the effect of Yangxintongmai tablet (YB) on the expression of VEGE and the numbers of new vessels in rabbits with atherosclerosis (AS) and its mechanism.Methods Twenty-four rabbits were divided into control group, model group and YB group. The rabbits models were made by feeding food rich in fat. The double-distilled water was used for intragastric treatment to the rabbits in control group and the model group. The rabbits in YB group were treated by YB fluid. One month later, the aorta of the rabbits was analyzed by immunohistochemistry to observe VEGF and the numbers of new vessels.Results Yellow positive expressions in the aorta plaque were found in the model group and the YB group. The expression of model group was more intensive(P<0.01).Conclusion YB could reverse or subside the aorta plaque effectually after aorta plaque development by effecting the expression of VEGF and new vessels.