The buoyant density of synaptic plasma membranes from the cerebral cortex of neonatal rats |
| |
Authors: | R. L. Norman B. D. Howard |
| |
Affiliation: | (1) Present address: Department of Biochemistry, Scripps Clinic and Research Foundation, 476 Prospect St., 92037 La Jolla, CA;(2) Department of Biological Chemistry, UCLA School of Medicine, 90024 Los Angeles, CA, USA |
| |
Abstract: | Summary A fraction enriched in synaptic plasma membranes was prepared from neonatal (5–6 day old) rat cerebral cortex. The procedure was based on a method used to prepare synaptic plasma membranes from adult cerebral cortex. Critical steps were monitored by electron microscopy. Synaptic plasma membranes from neonatal cerebral cortex sedimented as a broad peak between 0.9 M and 1.1 M sucrose. In contrast, the majority of adult synaptic plasma membranes have been reported to sediment to 1.2 M sucrose. The activities of various enzyme markers were determined in subfractions of neonatal preparations in order to estimate contamination. The specific activities of these markers indicated substantial contamination of the neonatal synaptic plasma membrane fractions by microsomes and glia. |
| |
Keywords: | Cerebral cortex Synaptic plasma membrane Buoyant density |
本文献已被 SpringerLink 等数据库收录! |