大鼠局灶性脑缺血模型的有效制备

目的对川西亚种猕猴Mamu-A^＊01等位基因的存在情况进行初步筛查。方法随机筛选2007年出生的猕猴101只，股静脉采取抗凝血200μl，提取基因组。根据Mamu．A^＊01等位基因的特异性序列设计引物，利用序列特异性引物PCR（PCR—SSP）筛查101只猕猴Mamu—A^＊01等位基因的存在情况。结果从101只猕猴中筛选到6只携带Mamu—A^＊01等位基因的猕猴，但扩增所得到的序列与印度猕猴对应序列的同源性只有96％。结论在川西亚种猕猴群内存在Mamu—A^＊01类似等位基因，但与印度猕猴有一定的差异，其作用和功能需要进一步的研究。

Comparison of Three Procedures in the Establishment of Rat Models of Focal Cerebral Ischemia

Objective To investigate the Mamu-A^＊01 allele of Chinese rhesus monkey in our monkey colony. Methods The blood sample （200 μl） were collected from femoral vein of 101 monkeys borm in 2007 randomly selected from the colony and the genomic DNA was extracted. The primers were designed according to the specific sequence of Mamu-A^＊01. The allele was investigation by sequence specific primers polymerase chain reaction. Results Six monkey were selected that carried Mamu-A^＊01 allele among the 110 monkeys, but the homology was only 96% with the Indian rhesus monkey＇s corresponding sequnce. Conclusions The Mamu-A^＊01 allele exist in Chinese rhesus monkeys, but was different with the Indian rhesus monkey, further work should be done to elucidate the function of the Mamu-A^＊01 allele.