高血压患者外周血单核细胞载脂蛋白E基因的表达及血脂分析

目的探讨人外周血单核细胞载脂蛋白E（Apo E）基因表达状况及其与高血压、血脂的相关性。方法分离外周血单核细胞,采用RT PCR方法,分析120例标本外周血单核细胞Apo E基因表达,并测定其血脂总胆固醇(TC)、甘油三酯(TG) 高密度脂蛋白(HDL C)、低密度脂蛋白(LDL C)、载脂蛋白A1（apo A1）、载脂蛋白B100（apoB100）和脂蛋白α［Lp(α)］含量。结果Apo E基因可以在人外周血单核细胞表达,高危和极高危高血压患者外周血单核细胞Apo E基因表达水平较健康人明显下调（P＜0.05）,随着危险度的增高,下调更明显。Apo E基因表达水平与LDL C呈负相关（r=-0.979,P＜0.05）,与TC、TG、apoB100、apoA1、Lp(α)无明显相关性（P＞0.05）。结论高危和极高危高血压患者外周血单核细胞Apo E基因表达水平明显下调,并与血脂代谢异常相关联,提示Apo E基因表达水平变化与高血压的发生发展相关联。

Apolipoprotein E gene expression in peripheral blood monocyte and lipid analysis of hypertension patients

Objective To explore the expression of the apolipoprotein E(Apo E) gene in peripheral blood monocytes and its relationship with lipids and hypertension.Method Peripheral blood monocyte were isolated and Apo E gene expression was determined by the RT-PCR method,and also the lipid level was determined.Results The Apo E gene was expressed in peripheral blood monocytes,and it was lower-expressed in the high risk group and very high risk group(P<0.05).The higher the risk grade,the lower the Apo E gene expression...