乌拉坦影响红细胞能量代谢导致溶血反应的机制研究

目的探讨乌拉坦静脉注射,对红细胞的能量代谢的影响.方法 20只家兔使用25%乌拉坦经耳缘静脉麻醉,给药30 min后经颈外静脉采血.每只家兔血样使用肝素抗凝后,分为3组,分别加入葡萄糖(GS)、三磷酸腺苷(ATP)和氯化钠(Nac)l以观察溶血率是否可以被降低.结果实验后血样,葡萄糖(GS)组和三磷酸腺苷(ATP)组,与Nacl对照组相比,GS组溶血率明显下降(P<0.05);ATP组溶血率明显下降(P<0.01).并且与GS对照组相比,ATP组溶血率也明显下降(P<0.01).结论乌拉坦静脉注射后,红细胞破坏溶解现象可以被葡萄糖(GS)和三磷酸腺苷(ATP)纠正,并且加入ATP可使溶血率的下降更为明显.乌拉坦可能是影响了红细胞糖酵解过程中的某个环节及ATP的利用,造成红细胞能量供应不足,凋亡溶解.

The Effect of Urethane on Red Blood Cell Energy Metabolism and Hemolytic Reaction

Objective To investigate the effect of urethane on the energy metabolism of red blood cell.Method 20 rabbits were anesthesed by urethane intravenous injection.We got blood samples through external jugular vein after 30 min,and divided blood samples which after anesthesia into control group,GS group,and ATP group,then observed whether the hemolytic rate can be reduced.Results Compared with control group,the hemolytic rate in GS group was decreased(P<0.05).ATP group’s hemolytic rate was remarkably decreased(P<0.01).And compared with GS group,the hemolytic rate of ATP group was also decreased significantly(P < 0.01).Conclusions After anesthesia,the hemolytic rate is alleviated when we put glucose(GS)and adenosine triphosphate(ATP)into blood sample.And ATP is better than GS.Urethane probably affects the energy metabolism of red blood cells.It makes the glycolysis decreased.