钛氟金云母/磷灰石玻璃陶瓷涂层与成骨细胞生物相容性研究

目的:探讨氟金云母/磷灰石玻璃陶瓷涂层作为口腔种植材料的可行性.方法:将小鼠的成骨细胞与涂层有氟金云母/磷灰石玻璃陶瓷的纯钛进行体外复合培养,通过扫描电镜观察,上清液碱性磷酸酶活性测定和流式细胞仪细胞周期测定以明确氟金云母/磷灰石涂层对成骨细胞生物学性状的影响.结果:成骨细胞在氟金云母/磷灰石涂层表面生长状态良好,两种材料不同比例实验组(F5组和F8组)的上清液碱性磷酸酶活性和细胞增殖指数与对照组差异有统计学意义(P＜0.05).结论:氟金云母/磷灰石玻璃陶瓷涂层具有良好的生物相容性.成骨细胞在氟金云母/磷灰石涂层上较对照组有更高的细胞增殖率.氟金云母/磷灰石涂层可用于纯钛涂层作为口腔种植材料.

Study on biocompatibility of mica/apatite glass-ceramics coating on titanium and osteoblasts in vitro

Objective： To study the possibility of mica/apatite glass-ceramics biomaterial coating on titanium used as a dental implant material. Method： Osteoblasts of the mouse were cultivated with mica/apatite glass-ceramics biomaterial coating on titanium together in vitro. Biological properties affected with bioactive glass ceramics were observed and mea- sured by electron microscope, detection of alkaline phosphatase in supernatant liquid and flow cytometer. Result： The os- teoblasts grew well on the surface of the bioactive glass ceramics, and there were significant differences of ALP in supernatant liquid between experimental and control groups （P〈0.05）. Conclusion： Mica/apatite glass-ceramics biomaterial coating was shown to possess good biocompatibility and bioactivity. Cells mc3t3el showed higher proliferation rate on the surface of coating than in the control group. The titanium alloy coated with mica/apatite glass-ceramics was confirmed to have great potential to be used as bone substitution material.