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| 诱导型一氧化氮合酶在博莱霉素致大鼠肺纤维化中的作用研究 | |
| 引用本文： | 李艳荣,刘睿,赵珍,刘萍,张丹丹,王殿华.诱导型一氧化氮合酶在博莱霉素致大鼠肺纤维化中的作用研究[J].昆明医学院学报,2012,33(3):22-25. |
| 作者姓名： | 李艳荣 刘睿 赵珍 刘萍 张丹丹 王殿华 |
| 作者单位： | 1. 昆明医学院云南省天然药物药理重点实验室,云南,昆明,650031 2. 云南省第一人民医院麻醉科,云南,昆明,650034 3. 昆明医学院学报编辑部,云南,昆明,650031 |
| 基金项目： | 云南省昆明医学院博士创新基金资助项目 |
| 摘 要： | 目的研究诱导型一氧化氮合酶(iNOS)在大鼠肺纤维化中的作用.方法在博莱霉素(BLM)致大鼠肺纤维化模型上,用免疫组化染色法测定肺组织iNOS的表达,硝酸还原酶法测定肺组织匀浆中NO含量,碱水法测定肺组织匀浆中HYP含量,HE染色法光镜观察肺组织形态学变化,评价iNOS在大鼠肺纤维化形成中的作用.结果肺组织NO含量:BLM组大鼠肺组织中NO含量高于对照组(P<0.05),L-NMMA组大鼠肺组织NO含量低于BLM组(P<0.05).肺组织HYP含量:BLM组大鼠肺组织中HYP含量高于对照组(P<0.05),L-NMMA组大鼠肺组织中HYP含量低于BLM组(P<0.05).肺组织iNOS表达:BLM组大鼠肺组织iNOS表达明显高于对照组(P<0.05);L-NMMA组大鼠肺组织iNOS表达低于BLM组(P<0.05).肺病理形态学:对照组大鼠肺组织结构正常;BLM组肺组织出现肺泡炎症、肺间质胶原纤维增生和肺纤维化;L-NMMA组大鼠肺部炎症和肺纤维化程度明显减轻.结论 iNOS抑制剂L-NMMA在一定程度能减轻肺炎症反应和肺纤维化程度,iNOS在肺纤维化中起着重要作用. |
| 关 键 词： | 大鼠 肺纤维化 诱导型一氧化氮合酶 |
Role of Inducible Nitric Oxide Synthase in Bleomycin- induced Pulmonary Fibrosis in Rats |
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| LI Yan-rong , LIU Rui , ZHAO Zhen , LIU Ping , ZHANG Dan-dan , WANG Dian-hua.Role of Inducible Nitric Oxide Synthase in Bleomycin- induced Pulmonary Fibrosis in Rats[J].Journal of Kunming Medical College,2012,33(3):22-25. | |
| Authors: | LI Yan-rong LIU Rui ZHAO Zhen LIU Ping ZHANG Dan-dan WANG Dian-hua |
| Institution: | 1)(1)Dept.of Yunnan Key Laboratory of Pharmacology for Natural Products,Kunming Medical University,Kunming Yunnan 650031;2)Dept.of Anesthesiology,The 1st People’s Hospital of Yunnan Province,Kunming Yunnan 650034;3)Dept.of Journal Editorial Board,Kunming Medical University, Kunming Yunnan 650031,China) |
| Abstract: | Objective To study the role of inducible nitric oxide synthase in Bleomycin-induced pulmonary fibrosis in rats.Methods We used the pulmonary fibrosis model induced by bleomycin(BLM)to evaluate the role of iNOS in the formation process of pulmonary fibrosis in rats.The expression of iNOS in lung tissue was determined by the immunohistochemical staining method.The NO levels in the lung homogenates were measured by the nitrate reductase assay.The HYP content in lung homogenates was evaluated by the alkaline method.The lung tissue morphological changes were observed by light microscopy after HE staining.Results In the NO levels of the lung tissue,BLM group was higher than the control group(P<0.05)and L-NMMA group was lower than the BLM group(P<0.05).In the HYP levels in the lung tissue,BLM group was higher than the control group(P<0.05)and L-NMMA group was lower than the BLM group(P<0.05).In the iNOS expression of the lung tissue,BLM group was obviously higher than the control group(P<0.05)and L-NMMA group was lower than the BLM group(P<0.05).In the pathological morphology of the lung,the lung tissue was normal in rats in the control group,the lung tissue appeared alveolar inflammation,pulmonary interstitial collagen increase and pulmonary fibrosis in BLM group and the lung inflammation and pulmonary fibrosis in L-NMMA group was reduced significantly.Conclusion L-NMMA,a iNOS inhibitor,can reduce in a certain extent the inflammatory response of lung tissue and pulmonary fibrosis,suggesting iNOS plays an important role in the formation of pulmonary fibrosis |
| Keywords: | Rats Pulmonary fibrosis Inducible nitric oxide synthase |
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