嗜酸粒细胞性支气管炎与咳嗽变异性哮喘患者的气道炎症特征

目的研究嗜酸粒细胞性支气管炎以及咳嗽变异性哮喘患者气道炎性细胞因子以及炎性介质的特性,明确二者的不同气道炎症特性的潜在机理.方法 EB组16例患者,CVA组16例患者、哮喘组15例患者以及对照组15名正常体检者,检测这4组人员诱导痰中的嗜酸粒细胞即EOS比例;流式检测IL-5和γ-IFN刺激的EOS表面CD69蛋白的表达;ELISA法检测各组患者诱导痰上清液中PGE2、LTC4的蛋白表达量.结果 EB组、CVA组、哮喘组患者诱导痰中EOS表面CD69蛋白的表达量差异不具统计学意义,与对照组相比具有统计学意义(P<0.05).EB组与对照组、CVA组、哮喘组患者诱导痰中PGE2浓度比较差异有统计学意义(P<0.05);与对照组相比,CVA组患者、EB患者以及哮喘组患者的诱导痰中LTC4浓度显著增高(P<0.05),EB组患者的LTC4的蛋白表达量和CVA组患者以及哮喘组患者差异有统计学意义(P<0.05).结论 EB组患者的诱导痰中PGE2的表达水平增高,CVA组患者的LTC4/PGE2比值与EB组患者相比也是明显升高,推测这2个因素是EB缺乏气道反应性的潜在炎症基础.

The Characteristics of Airway Inflammation in Eosinophilic Bronchitis and Cough Variant Asthma

Objective To study the characteristics of inflammatory cytokines in eosinophilic bronchitis and cough variant asthma.Methods EB group of 16 patients,CVA group of 16 patients,15 cases of asthma patients and normal control group of 15 healthy subjects,the percentage of EOS was detected by flow cytometry.EOS surface CD69 protein expression stimulated by IL-5 and γ-IFN was detected by flow cytometry.PGE2,LTC4 protein expression in induced sputum supernatant of patients was detected by ELISA.Results EOS surface expression of CD69 protein in induced sputum in EB group,CVA group and asthma group had no statistically significant difference,but there was a statistically significant difference in comparison with control group(P <0.05).Compared with PGE2 concentration of control group,there was statistically significant differences among EB group,CVA group,and asthma patients.Compared with control group,the concentration of LTC4 in CVA patients,EB patients and asthma patients was significantly higher(P <0.05).The protein expression of LTC4 in EB patients was higher than CVA patients and asthmatic patients(P <0.05).The ratio of LTC4/PGE2 in CVA patients and asthmatic patients was higher than EB group(P <0.05).Conclusions The expression levels of PGE2 increase in sputum of patients in EB group.Compared with the EB group,LTC4 / PGE2 ratio in CVA groupis significantly higher.