| HPLC法测定大鼠血浆中甲硝唑的浓度 |
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| 引用本文： | 赵春梅,杨和金,苏梅,蔡曼玲,徐红花,毛勇.HPLC法测定大鼠血浆中甲硝唑的浓度[J].昆明医学院学报,2012,33(5):40-42. |
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| 作者姓名： | 赵春梅 杨和金 苏梅 蔡曼玲 徐红花 毛勇 |
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| 作者单位： | 1. 云南省药物研究所,云南,昆明,650111
2. 昆明市第一人民医院,云南,昆明,650011 |
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| 基金项目： | 云南省院省校科技合作计划基金资助项目 |
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| 摘 要： | 目的 建立大鼠血浆中甲硝唑浓度测定的HPLC法.方法 选用Agilent Zorbax Eclipse XDB-C18色谱柱,流动相为甲醇-乙腈-水-冰乙酸(10:10:80:0.1),流速为1.0 mL/min,紫外检测波长为317nm,柱温25℃,以替硝唑作内标物.结果 甲硝唑的线性范围为1~1 000mg/L,呈良好的线性关系(r=0.996 9),在低、中、高3个质量浓度的准确度分别为99.69%、96.35%、101.2%.RSD分别为0.3%、6.92%、8.66%,其日内、日间精密度≤15%.结论 可用于甲硝唑在大鼠体内的药物动力学研究.
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| 关 键 词： | 甲硝唑 大鼠 血浆 药物动力学 高效液相色谱 |
Determination of Metronidazole in Rat Plasma by High-performance Liquid Chromatography |
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| ZHAO Chun-mei
,
YANG He-jin
,
SU Mei
,
CAI Man-ling
,
XU Hong-hua
,
MAO Yong.Determination of Metronidazole in Rat Plasma by High-performance Liquid Chromatography[J].Journal of Kunming Medical College,2012,33(5):40-42. |
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| Authors: | ZHAO Chun-mei YANG He-jin SU Mei CAI Man-ling XU Hong-hua MAO Yong |
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| Institution: | 1)(1)Yunnan Institute of Materia Medica,Kunming Yunnan 650111;2)The First People’s Hospital of Kunming,Kunming Yunnan 650011,China) |
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| Abstract: | Objective To develop a method for determination of metronidazole and tinidazole in rat plasma.Methods A Agilent Zorbax Eclipse XDB-C18 column was used at column temperature of 25 ℃,and the mobile phase contained methanol-acetonitrile-water-acetic-acid(10:10:80:0.1,V:V),with tinidazole as the internal standard.The analyte was determined by UV at 317 nm.Results Metronidazole show good relation at range of 1～1 000 mg/L(r =0.9969),the accuracy of metronidazole were 99.69%,96.35%,101.2%.RSD were 0.3%,6.92%,8.66%.The precision RSD was less than 15 %.Conlcusions The method has been proved to be a simple and sensitive method with good precision and accuracy.It is suitable for studying pharmacokinetics of metronidazole in rats |
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| Keywords: | Metronidazole Rat Plasma Pharmacokinetics HPLC |
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