响应面法优化微波提取小香薷籽总黄酮工艺及数学模型研究

目的 利用响应面法优化小香薷籽总黄酮工艺.方法 在单因素实验的基础上,选择乙醇浓度、预浸时间、料液比、微波提取时间4个主要影响因素,以总黄酮得率为响应值,采用响应曲面法中的Box-Behnken模式对提取工艺进行研究.结果 最佳工艺条件为乙醇浓度97.51%,预浸时间5.34 min,料液比1:47.5,微波时间5.76 min,总黄酮提取含量预测值为6.67%.结论 此条件下小香薷籽总黄酮提取含量为6.42%,与预测值比较接近,误差为3.7%;优选得到的工艺稳定可行,可为工业生产提供参考依据.

Study on Technique Condition of Extracting Total Flavones from Micromeria Barosma(W.W.Smith)Hand.-Mazz.Seed via Response Surface Methodology Optimizing Microwave and Its Mathematical Model

Objective To optimize the extraction technology of total flavonoids from Micromeria barosma(W.W.Smith)Hand.-Mazz.Seed.Methods Based on the single factor analysis,ethanol concentration,presoaking time,solid-liquid ratio to liquid and microwave heating time were chosen as main factors to evaluate the effects on the extraction of the total flavonoids.Results The optimized extraction conditions were as follows: ethanol concentration 97.51%,presoaking time 5.34 min,solid-liquid ratio 1:47.5,microwave heating time 5.76 min.The predictive value of total flavonoid content was 6.67%.Conclusions Under the optimal conditions,the yield of total flavonoids was 6.42%,close to predictive value error =3.7%.The extraction technology is feasible and stable and could be helpful to the factory production and better than orthogonal design.