结合珠蛋白mRNA及其蛋白在尖锐湿疣皮损中的表达

目的:从mRNA和蛋白水平检测尖锐湿疣皮损中结合珠蛋白的表达,探讨结合珠蛋白在尖锐湿疣皮损局部免疫异常机制中的作用.方法:采用原位杂交、免疫组织化学技术和Western免疫印迹的方法,检测30例尖锐湿疣患者皮损及20例正常包皮组织中结合珠蛋白mRNA及其蛋白的表达情况.结果:结合珠蛋白mRNA及其蛋白表达部位主要在表皮的基底层和棘细胞层.结合珠蛋白mRNA在尖锐湿疣皮损组和对照组中原位杂交染色灰度值分别为106.24±5.85和122.72±4.41,差异有统计学意义(P＜0.05);结合珠蛋白在尖锐湿疣皮损组和对照组中免疫组化染色灰度值分别为120.24±1.83和128.72±2.41,差异有统计学意义(P＜0.05);Western免疫印迹检测结果显示尖锐湿疣皮损组和对照组光密度值分别为0.53±0.11和0.24±0.06,尖锐湿疣皮损组表达较对照组明显升高(P＜0.05).结论:尖锐湿疣皮损表皮细胞中结合珠蛋白mRNA表达增强,合成结合珠蛋白水平升高.尖锐湿疣皮损中结合珠蛋白可能通过抑制朗格汉斯细胞的功能成熟和抑制角质形成细胞的免疫活性参与尖锐湿疣的局部免疫逃逸.

mRNA and protein expression of haptoglobin inlesions of condyloma acuminatum

Objective To investigate the expression of haptoglobin in the lesions of condyloma acuminatum(CA)at the mRNA and protein level,and to explore its role in the pathogenesis of CA.Methods The expressions of haptoglobin protein and mRNA in the skin tissues of 30 patients with CA and 20 normal controls were detected by immunohistochemistry(IHC),Western blot,and hybridization in situ.Results The in situ hybridization study showed that haptoglobin mRNA was expressed in the epidermal cells in the lesions of CA.The distribution of haptoglobin mRNA expression in the lesions of CA was similar to that of the normal controls,and the expression of haptoglobin mRNA in CA was higher than that of the nomal controls.There was a significant difference in the positive expression of haptoglobin mRNA between the CA group and the control group(P<0.05).The immunohistochemical study showed that haptoglobin protein was expressed in the whole layers of epidermal keratinocytes in the lesions of CA at a high level and stronger staining was seen in the stratum basale and stratum spinosum.Haptoglobin protein was expressed predominantly in the stratum basale in normal skin tissues,while weak staining was seen below the stratum spinosum.There was a significant difference in the mean gray value between the CA group and control group(P<0.05).Western blot showed that the haptoglobin expression in CA lesions significantly increased compared with the normal skins(P<0.05).Conclusion The expression of haptoglobin mRNA in the CA lesions obviously increases and the epidermal cells in the CA lesions are able to synthesize haptoglobin protein.Haptoglobin in the CA lesions may invovle in the local immunity escape by preventing Langerhans cell functional maturation and inhibiting the immunocompetence of keratinocyte.