HPLC法测定注射用12种复合维生素中维生素D_2、维生素A与维生素E的含量

目的:建立以高效液相色谱法同时测定注射用12种复合维生素中维生素D2、维生素A与维生素E含量的方法。方法:色谱柱为Inertsil(ODS-2)C18,流动相为乙腈-甲醇-二氯甲烷(80∶10∶10) ,流速为1mL·min-1,检测波长为265nm,进样量为10μL,柱温为30℃。结果:维生素D2、维生素E醋酸酯与维生素A棕榈酸酯检测浓度的线性范围分别为0.61～1.43、222.6～519.4μg·mL-1和1.21～2.82mg·mL-1;平均回收率分别为99.79%、99.53%、101.63%,RSD分别为1.667%、0.184 6%、0.113 4%。结论:本方法简单、快速、准确,可用于复合维生素制剂的含量测定。

Determination of Vitamin D2, Vitamin A and Vitamin E in Cernevit- 12 for Injection by HPLC

OBJECTIVE: To establish a HPLC method for the simultaneously determination of Vitamin D2, Vitamin A and Vitamin E in Cernevit-12 for injection. METHODS: An Inertsil(ODS-2) C18 column was selected as separation column at 30℃. The mobile phase consisted of acetonitrile-methanol-methylene chloride (80∶10∶10) at a flow rate of 1.0mL·min-1. The detection wavelength was set at 265nm and the injection volume was 10μL. RESULTS: The linear ranges of Vitamin D2, Vitamin E acetic ester and Vitamin A palmitate were 0.61～1.43μg·mL-1, 222.6～519.4μg·mL-1 and 1.21～2.82mg·mL-1 respectively. The average recoveries were 99.79%, 99.53% and 101.63%, with RSD of 1.66%, 0.184 6%, 0.113 4%. CONCLUSION: The method is simple, rapid and accurate, and suitable for the determination of Cernevit-12.