| BMSCs移植对大鼠脑梗死后神经功能恢复及Nogo-A表达的影响 |
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| 引用本文： | 郝光,杨凤刚,冯念苹,金永华,梁松岚. BMSCs移植对大鼠脑梗死后神经功能恢复及Nogo-A表达的影响[J]. 卒中与神经疾病, 2011, 18(5): 259-262,274. DOI: 10.3969/j.issn.1007-0478.2011.05.001 |
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| 作者姓名： | 郝光 杨凤刚 冯念苹 金永华 梁松岚 |
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| 作者单位： | 哈尔滨医科大学附属第二医院神经内科,哈尔滨,150000 |
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| 摘 要： | 目的探讨骨髓间充质干细胞（Bone Marrow-derived mesenchymal stem cells,BMSCs）静脉移植对大鼠脑梗死后神经功能恢复及对Nogo-A表达的影响。方法将大鼠随机分成假手术组、模型对照组、溶剂对照组和移植组。全骨髓贴壁法分离培养大鼠BMSCs,线栓法制作大鼠MCAO模型;移植组自尾静脉注射BMSCs悬液,溶剂对照组注射磷酸盐缓冲液;造模成功后第3、7、14和21 d通过神经功能缺损程度评分,观察其恢复状况;RT-PCR法检测Nogo-AmRNA的表达水平,免疫组化方法检测Nogo-A蛋白的表达水平。结果移植组第7、14和21 d神经功能恢复优于对照组;移植组第3、14和21 d脑组织损伤区周边组织中Nogo-AmRNA表达水平和Nogo-A蛋白的表达水平较对照组降低（P〈0.05）。结论 BMSCs移植可促进大鼠脑梗死后的神经功能恢复,其作用机制可能与下调Nogo-A的表达水平有关。
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| 关 键 词： | 骨髓间充质干细胞 脑梗死 Nogo-A |
Effect of bone marrow-derived mesenchymal stem cells transplantation on the expression of Nogo-A in rats with cerebral infarction |
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| Hao Guang,Yang Fenggang,Feng Nianping,et al.. Effect of bone marrow-derived mesenchymal stem cells transplantation on the expression of Nogo-A in rats with cerebral infarction[J]. Stroke and Nervous Diseases, 2011, 18(5): 259-262,274. DOI: 10.3969/j.issn.1007-0478.2011.05.001 |
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| Authors: | Hao Guang Yang Fenggang Feng Nianping et al. |
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| Affiliation: | Hao Guang,Yang Fenggang,Feng Nianping,et al.Department of Neurology,the Second Affiliated Hospital of Harbin Medical Vniversity,Marbin 150000 |
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| Abstract: | Objective To investigate the effects of intravenous administration of bone marrow-derived mesenchymal stem cells(BMSCs)on neurological functional recovering and the expressions of Nogo-A in rats with cerebral infarction. Methods Rats were randomized into 4 groups:sham operation group,MCAO group,Vehicle group and BMSCs group.BMSCs were harvested and purified following adherence technique.All rats were subjected to transient(2-hour) middle cerebral artery occlusion.BMSCs were injected into tail vein in BMSCs ... |
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| Keywords: | Bone marrow-derived mesenchymal stem cells Cerebral infarction Nogo-A |
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