| 慢性束缚应激对大鼠体质量、血清皮质酮和前额叶皮质cAMP-PKA-CREB-BDNF通路的影响 |
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| 引用本文： | 孟珊珊,刘洪文.慢性束缚应激对大鼠体质量、血清皮质酮和前额叶皮质cAMP-PKA-CREB-BDNF通路的影响[J].卒中与神经疾病,2011,18(5):263-265,270. |
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| 作者姓名： | 孟珊珊 刘洪文 |
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| 作者单位： | 哈尔滨医科大学附属第一医院大桥分院,150076 |
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| 摘 要： | 目的探讨慢性束缚应激对大鼠体质量、血清皮质酮水平和前额叶皮质cAMP-PKA-CREB-BDNF通路的影响。方法将30只雄性SD大鼠随机分为对照组、慢性束缚应激1周组和慢性束缚应激2周组,每组各10只。比较应激前后大鼠体质量和血清皮质酮水平的变化,放免法观察应激前后大鼠前额叶皮质环磷腺苷(cAMP)含量,试剂盒测定cAMP依赖蛋白激酶(protein kinase,PKA)活性和脑源性神经营养因子(brain derived neurophic factor,BDNF)表达水平,用蛋白免疫印迹技术观察cAMP反应元件结合蛋白质(p-CREB)水平变化。结果慢性束缚应激组大鼠在应激1周和应激2周的体质量增长分别为(35±11)g和(23±8)g]均低于对照组分别为(59±16)g和(68±14)g](P<0.05)。束缚应激1周组和束缚应激2周组大鼠血清皮质醇浓度分别为(356.78±112.21)ng/ml、(558.49±98.32)ng/ml,均高于对照组(198.21±101.35)ng/ml(P<0.05,P<0.01)。束缚应激1周组和束缚应激2周组大鼠前额叶皮质的cAMP含量、PKA活性、p-CREB和BDNF表达水平低于对照组(P<0.01)。结论慢性束缚应激抑制大鼠体质量的增长,引起血清皮质酮水平增高和前额叶皮质cAMP-PKA-CREB-BDNF信号通路被抑制。
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| 关 键 词： | 束缚应激 体质量 皮质酮 前额叶皮质 cAMP反应元件结合蛋白质 |
Effect of chronic restraint stress on body weight,serum corticosterone concentration and cAMP-PKA-CREB-BDNF signaling activity in prefrontal cortex in rats |
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| Meng Shanshan,Liu Hongwen.Effect of chronic restraint stress on body weight,serum corticosterone concentration and cAMP-PKA-CREB-BDNF signaling activity in prefrontal cortex in rats[J].Stroke and Nervous Diseases,2011,18(5):263-265,270. |
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| Authors: | Meng Shanshan Liu Hongwen |
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| Institution: | Meng Shanshan,Liu Hongwen.Daqiao Department,the First Affiliated Hospital of Harbin Medical University,Haerbin 150076 |
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| Abstract: | Objective To observe effect of chronic restraint stress on body weight,serum corticosterone concentration and cAMP-PKA-CREB-BDNF signaling activity in prefrontal cortex in the rat. Methods Thirty male SD rats were randomly divided into control group(n=10),1 week and 2 weeks(n=10) restraint stress groups.Before and after restraint stress,body weight and serum corticosterone concentration were examined,radio-immunity method Pep TagR detecting system,and western blotting were used to assess cAMP contert.PKA ac... |
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| Keywords: | Restraint stress Body weight Corticosterone Prefrontal cortex cAMP response element binding protein |
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