胰腺癌患者血清相关蛋白质差异表达的研究

目的 利用肿瘤血清蛋白质组分析方法 (serologic proteome analysis,SERPA)对胰腺癌患者血清和正常人血清进行蛋白质组的分析,寻找胰腺癌特异的肿瘤标记物.方法 利用HPLC柱去除血清中的自蛋白,通过双向电泳分离蛋白质,经图像采集与分析,切取胰腺癌患者血清和正常人血清的差异蛋白质点,进行质谱分析鉴定.结果 共获得4个差异蛋白质.正常人血清含量高而胰腺癌患者血清含量低的蛋白质为胍裂解环化酶激活蛋白2(guanylate cyclase-activating protein 2).胰腺癌患者血清含量高而正常人血清含量低的蛋白质3个,分别为结合珠蛋白2α(haptoglobin 2 alpha)、转甲状腺素蛋白(transthyretin)及KIAA1018蛋白.结论KIAA1018有望成为胰腺癌筛选和早期诊断的肿瘤标记物.

Differential expression of serum proteins of pancreatic cancer patients

Objective Serologic proteome analysis method (SERPA) was used to compare the different of the serum proteins between normal and pancreatic cancer patients' serum, and to find a new specific pancreatic cancer marker. Methods HPLC was used to eliminate albumin from the serum, two-dimensional electrophoresis was used to separate the proteins. After imaging collection and analysis, the different proteins between pancreatic cancer and normal subjects were cut for mass spectrometry. Results Four discrepancy proteins were obtained. Guanylate cyclase-activating protein 2 was highly expressed in normal serum but not pancreatic cancer. Hp2-alpha, transthyretin and KIAA1018 protein were highly expressed in cancer patients'serum but not normal people. Conclusions KIAA1018 may become a promising tumor marker for screening and early diagnosis of pancreatic cancer.