新癀片对急性胰腺炎大鼠胰腺细胞凋亡的影响

目的 观察新癀片对急性胰腺炎(AP)大鼠胰腺细胞凋亡和胰腺组织Bcl-2、c-Myc蛋白表达的影响,并探讨其作用机制.方法 90只Wistar大鼠按完全随机法分为对照组、AP组和新癀片治疗组.采用胆胰管逆行注射5%牛黄胆酸钠1 ml/kg体重方法制备AP模型.治疗组于制模后30 min按480 mg/kg体重的剂量予新癀片水溶液灌胃.术后6、12、24 h分批处死大鼠,检测血淀粉酶活性,观察胰腺病理变化,TUNEL法检测胰腺细胞凋亡,Western blotting法检测胰腺组织Bcl-2、c-Myc蛋白表达.结果 对照组12 h点的血淀粉酶活性、胰腺病理评分、凋亡指数、胰腺组织Bcl-2和c-Myc蛋白表达量分别为(1927±186)U/L、0、0.59±0.12、0.406±0.112、0.185±0.046;AP组分别为(5611±473)U/L、2.65±0.56、3.80±0.91、0.282±0.082、0.339±0.076;治疗组分别为(4572±400)U/L、2.40±0.75、7.15±0.86、0.220±0.068、0.302±0.090.AP组和治疗组的淀粉酶活性、胰腺病理评分、凋亡指数、c-Myc蛋白表达量均显著高于对照组(P值均＜0.05),而Bcl-2蛋白表达量显著低于对照组(P＜0.05).与AP组比较,治疗组的淀粉酶活性、胰腺病理评分、胰腺组织Bcl-2蛋白表达量均显著降低(P值均＜0.05),凋亡指数显著增高(P＜0.05),c-Myc蛋白表达量无显著变化.结论 新癀片可通过下调Bcl-2蛋白表达、诱导AP早期胰腺细胞凋亡,从而减轻胰腺炎症反应.

Effect of Xinhuang Pian on pancreatic apoptosis in rat with acute pancreatitis

Objective To investigate the effect of Xinhuang Pian on pancreatic apoptosis and the protein expression of Bcl-2 and c-Myc in rat with acute pancreatitis (AP). Methods 90 Wister rats were randomly divided into 3 groups, including control group, AP group and Xinhuang Pian treatment (XP) group.AP model was induced by pancreaticobiliary injection of 5% sodium taurocholate (1ml/kg weight). Rats in XP group were given Xinhuang Pian solution (480 mg/kg weight) lavage 30min after AP induction, The rats were sacrificed at 6, 12, 24 h after AP induction, and serum amylase levels were determed, pancreatic pathological change was observed; apoptosis was determined by TUNEL method. The determination of Bcl-2and c-Myc protein expression was performed by Western blot. Results 12 h after AP induction, serum amylase, pancreatic pathological scores, apoptotic indexes and the protein expression of Bcl-2 and c-Myc were (1927 ± 186) U/L, 0, 0.59 ± 0. 12, 0.406 ± 0. 112, 0. 185 ± 0. 046 in control group; (5611 ± 473) U/L,2.65 ± 0. 56, 3.80 ± 0. 91, 0. 282 ± 0.082, 0. 339 ± 0. 076 in AP group; and (4572 ± 400) U/L, 2.40 ± 0. 75,7.15 ± 0. 86, 0. 220 ± 0.068, 0. 302 ± 0. 090 in XP group. The levels of serum amylase, pancreatic pathological scores, apoptotic indexes and the protein expression of c-myc in AP and XP group were all significantly higher than those in control group (P < 0. 05), while the protein expression of Bcl-2 was significanfiy lower than that in control group ( P < 0.05 ). Compared with AP group, the levels of serum amylase, pancreatic pathological scores, protein expression of Bcl-2 were all significanfiy lower in XP group (P < 0. 05 ), but the apoptotic indexe was significantly higher (P < 0. 05 ), and there was no significat change of expression of c-Myc protein.Conclusions Xinhuang Pian may induce the pancreatic apoptosis through down-regulating Bcl-2 protein expression, and result in the alleviation of pancreatic inflammatory reaction.