LRIC1基因在人星形细胞瘤中的表达与意义(英文)

目的 LRIG1基因是新发现的与果蝇 Kek-1基因同源的人类基因。Kek-1基因编码一种果蝇细胞表面蛋白—kekkon-1蛋白,它可抑制果蝇表皮细胞生长因子受体(EGFR)介导的信号转导。人类 LRIG1蛋白是一种跨膜糖蛋白,胞外区含亮氨酸重复区域和免疫球蛋白样区域。既往的研究显示 LRIG1几乎表达在所有被检测的人类组织中,如前列腺、乳腺、回肠、胃、肺和脑组织等。然而,目前对它在人类组织中的作用仍然未知。本研究的目的是进一步扩大 LRIG1的表达谱,并探讨它在肿瘤中的作用与意义。方法设计、制作 LRIG1 mRNA 寡核苷酸探针,用原位杂交检测 LRIG1 mRNA 在16例不同级别人星形细胞瘤中的表达,并与相应的肿瘤周边组织相比较;原代培养11例星形细胞瘤细胞,用 PCNA 行免疫组化及 LRIG1探针行原位杂交,分析培养细胞 PCNA 表达和 LRIG1表达之间的关系。结果人星形细胞瘤与其周边组织中均有一定程度的 LRIG1表达,肿瘤组织表达 LRIG1的水平较肿瘤周边组织低,这种降低的程度与肿瘤的级别成正比;培养细胞中也有一定程度的 LRIG1表达,其表达的程度与 PCNA 染色呈反比。结论 LRIG1蛋白具有抑制星形细胞瘤生长或增殖的作用,LRIG1的表达下调,可能参与了肿瘤的发生和发展过程。

Expression and Significance of LRIG1 Gene in Human Astrocytomas

Objective:LRIG1 gene is a newly found human gene that displays homologies to the Drosophila Kek-1 gene.Previous researches have shown that the LRIG1 gene almost expressed in all human tissues. However,its functions,particularly its functions in human tumors,are still unknown.The goals of the present study are to magnify the expression spectrum of the LRIG1 gene and determine their roles in the oncogenesis.Methods:A triphasic oligonucleotide probe was designed and used to detect the expression level of the LRIG1 gene in 16 astrocytomas and the corresponding tissues around the tumors by in situ hybridization.11 primary astrocytoma cells were cultured.Among these,the expression level of the LRIG1 gene was checked by in situ hybridization and the expression of the Proliferating Cell Nuclear Antigen (PCNA)protein was detected by immunohistochemistry.Results:The expression of LRIG1 protein was detected in different degree in all the tumors and the surrounding tissues.Compared to the surrounding tissues,the expression of the tumors was lower.The decrease extends from the surrounding tissues to the tumors were correlation to the tumors'grades.The primary cultured cells also expressed LRIG1 to various extent and the expression of LRIG1 in the cultures was negatively correlated with the intensity of the PCNA staining.Conclusion:The LRIG1 protein may inhibit the growth of tumors of glial cells and the down-regulation of the LRIG1 gene may be involved in the development and progression of the tumor.