类风湿关节炎患者滑膜抗环瓜氨酸肽 表位表达与肽酰基精氨酸脱亚氨酶4基因的相关性分析

目的 探讨类风湿关节炎患者滑膜抗环瓜氨酸肽(CCP)表位表达与肽酰基精氨酸脱亚氨酶4(PADI4)基因的相关性。方法 2013年2月～2015年选择在湖北医药学院附属东风医院风湿免疫科住院的类风湿关节炎患者110例作为观察组,同期选择在该院进行体检的健康者110例作为对照组,两组都进行滑膜抗CCP表位表达阳性率检测与PADI4基因表达检测,同时进行相关性分析。结果 观察组与对照组的滑膜抗CCP表位表达阳性率分别为70.9%和9.1%,观察组明显高于对照组(χ2=23.289,P<0.05)。观察组PADI4-104基因型的表达频率与对照组对比差异有统计学意义(χ2=2.984,P<0.05),多为G/G表达,而对照组多为C/G表达。Pearson相关性分析显示在观察组中,滑膜抗CCP表位阳性表达情况与PADI4-104基因型表达频率之间存在明显相关性(r=0.344,P<0.05); logistic逐步回归分析显示PADI4-104基因型表达频率是影响滑膜抗CCP表位表达的主要因素(P<0.05)。结论 类风湿关节炎患者的滑膜抗CCP表位表达阳性率明显增加,同时也存在PADI4基因多态性紊乱情况,两者也存在明显的相关性,从而影响类风湿关节炎的发病状况。

Relation Analysis of Synovial Anti-Citrullinated Epitope Peptide Expression and Peptidyl Arginine Deiminase 4 Gene in the Rheumatoid Arthritis Patient

Objective To investigate the relation of synovial anti-citrullinated epitope peptide(CCP)expression and peptidyl arginine deiminase 4(PADI4)gene in the rheumatoid arthritis patient.MethodsFrom February 2013 to 2015 in Dongfeng General Hospital Affiliated toHubei University,selected 110 patients with rheumatoid arthritis as the observation group,and selected 110 healthy peopleat the same period in this hospital for medical examination as the control group,both groups were given the synovial CCP expression positive rate and PADI4gene expression detecting and correlation analysis.Results The synovial anti-CCP expression positive rates in the observation group and the control group were 70.9% and 9.1% respectively,and the observation group were significantly higher than the control group(P<0.05).The PADI4-104genotype expression frequency compared between the observation group and control group,the difference were statistically significant(P<0.05).The G/G expression was more in the observation group,while the control group was more witthe C/G expression.Pearson correlation analysis showed that in the observationgroup,synovial CCP positive expression were significant correlated to the expression of genotype PADI4-104(r=0.344,P<0.05).Logistic regression analysis showed PADI4-104 genotype frequency were the main factors for the synovialCCP epitope expression(P<0.05).Conclusion The rheumatoid arthritis was more with synovial anti-CCP positive expression.There were also PADI4 polymorphism disorder expression,and clear correlation between the two.Thus impact the incidence of rheumatoid arthritis.