革兰阳性球菌对利奈唑胺耐药机制的研究

目的 对利奈唑胺耐药革兰阳性球菌主要耐药分子机制进行初步研究。方法 采用KB法及E-test法对6株利奈唑胺耐药革兰阳性球菌进行药敏试验,并应用脉冲场凝胶电泳(PFGE),PCR及测序技术对菌株主要分子流行病学及耐药分子机制进行研究。结果 5株柯氏葡萄球菌PFGE分为2型,对利奈唑胺MIC介于16～64 mg/L,菌株均呈现cfr基因阳性、L3存在双位点突变; 1株粪肠球菌对利奈唑胺MIC为8 mg/L,耐药与23SrRNA存在G2576T点突变相关。结论 利奈唑胺耐药革兰阳性球菌在临床的出现应引起广泛关注,为有效进行抗感染治疗应重视对利奈唑胺耐药菌株的临床监测。

Study on the Linezolid Resistance Mechanism of Gram Positive Cocci

Objective To study the molecular resistance mechanism of linezolid resistant gram positive cocci.Methods KB and E-test methods were used in antibiotic susceptibility tests.PFGE,PCRand sequencing techniques were applied to study the molecular epidemiological characteristics and resistance mechnisms of linezolid to gram positive cocci.Results The MICs of linezolid of the 5 Staphylococcus cohnii isolates were from 16 to 64 mg/L,and they were all cfr gene positive and with L3 mutations,and there were two PFGE types.The linezolid MIC of one Enterococcus faecalis was 8 mg/L with G2576T mutation in 23SrRNA.Conclusion There were linezolid resistant gram positive cocci appeared inclinical samples,for the effective anti-infection treatment monitoring linezolid resistant gram positive cocci in clinical lab is important.