血清淀粉样蛋白A(SAA)在儿童手足口病中的临床应用

目的 研究血清淀粉样蛋白A(SAA)在测定儿童手足口病中的作用并与相关的急性时相蛋白C反应蛋白(CRP)进行比对,以确定SAA在诊断儿童手足口病中的重要性。方法 随机选取陕西省人民医院门诊儿童手足口病标本96例和正常对照标本100例,两组血清淀粉样蛋白A用国产免疫比浊法试剂在日立7170A全自动生化仪上进行检测。结果 在96例儿童手足口病中SAA水平为425.82±120.30 ml/L,CRP水平为23.26±14.50 ml/L,两者阳性率分别为82.60%和65.21%。不同组人群血清SAA和CRP的结果、手足口病组与正常对照组比较,差异均有统计学意义(均P<0.01); 而SAA与CRP比较差异有统计学意义(P<0.01)。阳性率差异有统计学意义(χ2=8.962,P<0.01); 在手足口病组中的轻度、中度及重度患者SAA和CRP与对照组比较,差异均有统计学意义(χ2=9.132,P<0.01),而手足口病组中轻度、中度及重度患者之间SAA和CRP比较,SAA的轻度与中度,中度与重度之间比较,差异无统计学意义(χ2=0.706,P>0.05); CRP的轻度与中度、中度与重度、轻度与重度之间比较,差异均有统计学意义(χ2=6.316～9.262,P均<0.05或P<0.01)。结论 SAA的检测可作为儿童手足口病诊断的重要指标,特别是联合CRP检测对该疾病的早期诊断和预后提供重要的实验依据。

Clinical Application of Serum Amyloid A(SAA) in Children with Hand Foot and Mouth Disease

Objective To study the action of serum amyloidA(SAA)in children with hand foot and mouth disease(HFMD), and compare withan acute phase protein-C reactive protein(CRP),in order to determine the importance of the SAA on diagnosis of HFMD in children.Methods 96 clinical specimens with HFMD in children and 100 normal specimes were randomly selected in Shaanxi Provincial People's Hospitle.With domestic immunoturbidimetry assay reagent on Hitachi 7170A Automatic Biochemical Analyzer,the serum amyloid A concentration in the two groups were detected.ResultsIn 96 children with hand foot mouth disease,SAA concentration was 425.82±120.30 ml/L,CRP was 23.26±14.50 ml/L.The positive rates were 82.60% and 65.21%,respectively.Serum SAA and CRP results of different group,compared the HFMD group with normal control group,the differences were statisticallysignificant(P<0.01),and compared SAA with CRP,the differences was statistically significant(P<0.01).The positive rate of SAA was much higher than that of CRP,and the differences was statistically significant(χ2=8.962,P<0.01).In the HFMD group,SAA and CRP of mild,moderate and severe patients were compared with the control group,the differenses were statistically significant(χ2=9.132,P<0.01).However,in the HFMD group,compared SAA of mild with moderate and severe patients,there were no statistically significant difference(χ2=0.706,P>0.05),and compared CRP with moderate and severe patients,the differences were statistically significant(χ²=6.316～9.262,P<0.05 or P<0.01).Conclusion Thestudy results indicated that detection of SAA can be used as an important indexof diagnosis for children with HFMD,and provide basis for early diagnosis and prognosis of clinical with HFMD.