强直性脊柱炎患者外周血单个核细胞中Toll样受体表达及功能的研究

目的 研究强直性脊柱炎(ankylosing spondylitis,AS)患者外周血单个核细胞(peripheral blood mononuclear cells,PBMCs)中Toll样受体(toll like receptors,TLRs)的表达及功能,并探讨TLRs在AS发病中的作用。方法 通过荧光定量PCR的方法检测21例AS患者及21例健康对照者PBMCs上TLR1～TLR10的表达; 采用ELISA的方法检测AS患者及健康对照者PBMCs中TLRs介导的细胞因子产生水平,并检测患者及健康对照血浆中细胞因子的水平。结果 与对照组相比,AS患者组PBMCs中TLR4的表达水平显著升高,差异具有统计学意义(P=0.004),其他TLRs在两组间的表达差异无统计学意义; AS患者PBMCs中TLR4介导的肿瘤坏死因子α(TNF-α)和白细胞介素6(IL-6)的产生水平显著增高(P值分别为0.003和0.009); AS患者血浆中TNF-α的表达水平较健康对照组显著升高(P=0.021)。结论 AS患者PBMCs中TLR4的表达及细胞因子产生能力增强,TLR4通路可能在AS发病过程中起重要作用。

Expression Profiles and Function of Toll-like Receptors in Peripheral Blood Mononuclear Cells of Ankylosing Spondylitis Patients

Objective To study the expression profiles andfunction of Toll Like Receptors(TLRs)in peripheral blood mononuclear cells ofankylosing spondylitis(AS)patients,and investigate their function in pathogenesis of AS.Methods Quantitative analysis of TLR1-10 mRNA expression in PBMCs from 21 AS patients and 21 healthy controls by RT-qPCR.Investigated the ability of PBMCs from AS patients and healthy controls to produce cytokines in response to TLRs by ELISA,and also using ELISA to test the cytokine levels in the plasma of AS patients and healthy controls.ResultsThe expression of TLR4 in PBMCs was significantly increased in AS patients compared to healthy donors(P=0.004),and there was no statisticalsignificance for other TLRs expression between the two groups.After challenge with TLR4 ligands,significantly higher level of TNF-α and IL-6 production from PBMCs of AS patients were observed,compared with those from healthy donors(P=0.003,0.009).Also found that the level of TNF-α in plasma of AS patients was higher than healthy donors(P=0.021).Conclusion Study reveals the expression and function of TLR4 in PBMCs of AS patientswas higher than healthy controls,which means that TLR4 pathway may play an important role in pathogenesis of AS.