黏液型与非黏液型铜绿假单胞菌Cif基因表达研究

目的 比较黏液型和非黏液型铜绿假单胞菌囊性纤维化跨膜传导调节体抑制因子(Cif)基因表达差异,并结合临床资料比较对应患者白细胞(WBC)及C反应蛋白(CRP)等炎症指标的不同。方法 采用逆转录PCR和实时荧光定量PCR检测两种型别铜绿假单胞菌Cif基因的表达量。收集相应患者临床数据比较WBC数和CRP水平及其与Cif基因表达的相关性。结果 黏液型铜绿假单胞菌Cif基因表达量显著高于非黏液型铜绿假单胞菌,差异有统计学意义(t=2.09,P<0.05),黏液型铜绿假单胞菌组和非黏液型铜绿假单胞菌组的患者其WBC数和CRP水平差异无统计学意义(t=0.65,0.60,P>0.05); Cif基因表达与CRP水平相关性极弱(a=-0.061,R²=0.04),与WBC数相关性也极弱(a=0.095,R²=0.029)。结论 Cif基因表达可能与黏液型铜绿假单胞菌生物被膜的形成有关,可能为铜绿假单胞菌新的毒力因子。

Investigation of Cif Gene Expression between Mucoid and Non-mucoid Pseudonmonas Aeruginosa

Objective To compare the difference of the Cifgene expression and the inflammation markers(such as WBC and CRP et al)betweenmucoid and non-mucoid Pseudonmonas aeruginosa,combining with the clinicaldata.Methods Real-time RT-PCR was used to determine the expression level of Cif in Pseudonmonas aeruginosa and clinical data were collected to discuss the relationship among the number of WBC,the level of CRP and the Cif gene expression.Results The expression level of Cif gene in mucoid Pseudonmonas aeruginosa had a significantly higherthan the non-mucoid Pseudonmonas aeruginosa,which was statistically significant(t=2.09,P<0.05).Specimen data collected from patients with the infection of mucoid or non-mucoid Pseudonmonas aeruginosa showed that the number of WBC and the level of CRP were not significantly different(t=0.65,0.60,P>0.05).The data also showed that the cystic fibrosis transmembrane conductance regulator inhibitory factor(Cif)gene expression level had little correlation with the number of WBC(a=0.095,R²=0.029).Conclusion The expression of Cif gene may works for the biofilm formation of mucoid Pseudonmonas aeruginosa,which maybe a new virulence factor to Pseudonmonas aeruginosa.