In situ hybridization analysis of substance P receptor in the rat retina |
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Authors: | Akitoshi Kondoh Takeshi Houtani Teizo Ueyama Kazuyasu Baba Michiko Ikeda Kazuya Yamagishi Hirohiko Miki Masanobu Uyama Shigetada Nakanishi Tetsuo Sugimoto |
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Institution: | (1) Departments of Anatomy and Ophthalmology, Kansai Medical University, Moriguchi, 570 Osaka, Japan;(2) Department of Biological Sciences, Kyoto University, Faculty of Medicine, Sakyo-ku, 606-01 Kyoto, Japan;(3) Department of Anatomy, Kansai Medical University, Moriguchi, 570 Osaka, Japan |
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Abstract: | Substance P receptor is known to provide a principal interface between tachykinin peptides and tachykinin-sensitive cells in retinal circuitry and to produce several physiological functions such as excitation of ganglion cells. We reported results of in situ hybridization analysis of substance P receptor in rat retina using digoxigenin-labeled RNA probes to yield discrete cell labeling. Distinct hybridization signal was present in a great majority of ganglion cells that provide retinal fibers to a central target. It was also present in a subpopulation of amacrine cells. Following optic nerve crush, ganglion cells lost their hybridization signal in a time-dependent manner, while hybridization-positive amacrine cells were persistently seen. From the results, we identified the hybridization message as distinctly localized to two systems, output cells and intrinsic cells in retinal circuitry. |
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Keywords: | Tachykinin receptor Retrograde cell labeling Optic nerve crush Ganglion cell layer Inner nuclear layer Rat |
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