| 由DL-5-苯乙内酰脲酶法生产D-苯甘氨酸的菌种选育 |
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| 引用本文： | 马莉,齐秀兰,刘丽宏.由DL-5-苯乙内酰脲酶法生产D-苯甘氨酸的菌种选育[J].沈阳药科大学学报,1991(4). |
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| 作者姓名： | 马莉 齐秀兰 刘丽宏 |
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| 作者单位： | 沈阳药学院微生物教研室，沈阳药学院微生物教研室，沈阳药学院微生物教研室 53期微生物制药专业学生 |
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| 摘 要： | 本实验以假单孢菌Pseudomonas SP.27为出发菌株由DL-5-苯乙内酰脲发酵生产D-苯甘氨酸。野生菌产量为15.85μg/ml.首先对其进行紫外诱变处理1min,筛选出菌株Q_6,其产量为256.7μg/ml.然后用Q-6制备球形体,进行亚硝基胍诱变处理60min,得到Q_(68)菌株,其产量为453.4μg/ml.
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| 关 键 词： | DL-5-苯乙内酰脲 D-苯甘氨酸 球形体 |
SELECTION OF D-PHENYLGLYCINE PRODUCING STRAIN FROM DL-HYDANTOIN |
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| Ma Li Qi Xiulan Liu Lihong.SELECTION OF D-PHENYLGLYCINE PRODUCING STRAIN FROM DL-HYDANTOIN[J].Journal of Shenyang Pharmaceutical University,1991(4). |
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| Authors: | Ma Li Qi Xiulan Liu Lihong |
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| Institution: | Ma Li Qi Xiulan Liu Lihong Department of Microbiology |
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| Abstract: | Pseudomonas sp 27 was used as the starting strain to produce D-phenylglycine from DL-hydantoin in the experiment. Through fermentation, it produced 15.85μg/ml of D-phenylglycine. After the strain was treated with ultraviolet rays(UV) for 1 min, mutant Q6 was obtained, which produced 256.7μg/ml of D-phenylglycine. When spheroplast of mutant Q6 was treated for 60 min. with NTG, mutant Q68 was obtained, which produced 453.4μg/ml of D-phenylglycine. |
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| Keywords: | DL-5-Hydantoin D-Phenylglycine Spheroplast |
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