恒河猴肝移植后急性排斥反应中热休克蛋白70的早期诊断价值

背景：热休克蛋白是生物体在不利环境因素刺激下应激合成的一种特殊蛋白质，热休克蛋白70参与移植免疫反应并发挥重要作用。目的：探讨热休克蛋白70在恒河猴肝移植后急性排斥反应中的早期诊断价值。 方法：选用健康恒河猴采用改良二袖套+肝动脉重建的方法进行同种异体原位肝移植16例，移植后受体分为急性排斥组和对照组，每组8只，急性排斥组围手术期不行免疫抑制治疗，对照组围手术期使用免疫抑制治疗，分别在移植后6，12，24，72 h 4个时间点取移植后肝脏组织进行苏木精-伊红染色以判断排斥反应程度，western blotting检测肝组织中热休克蛋白70表达水平，免疫组化检测肝组织热休克蛋白70表达情况。结论与结论：肝移植后72 h时段内急性排斥组肝脏急性排斥反应的组织学表现重于对照组，Baff分级水平高于对照组(P < 0.05)，从移植后开始至移植后72 h两组移植肝经免疫组化和western blotting检测热休克蛋白70表达水平均有所升高，但急性排斥组要明显高于对照组(P < 0.05)。提示，未使用免疫抑制治疗的情况下，恒河猴肝移植后早期急性排斥反应在移植后72 h内即可明显观察到，热休克蛋白70在移植肝组织中表达水平也随着急性排斥反应的发生进展呈现明显上升趋势，对肝移植后早期急性排斥具有较高的预测和诊断价值。

Early diagnostic value of heat shock protein 70 in acute rejection after liver transplantation in rhesus monkeys

BACKGROUND: Heat shock protein 70 (HSP 70) is a special kind of protein by living organisms stress synthesized under the adverse environmental stimulation. It takes part and plays an important role in transplantation immunoreactions. OBJECTIVE: To investigate the early diagnostic value HSP 70 on acute rejection after liver transplantation in rhesus monkey.METHODS: Healthy rhesus monkeys were selected. A total of 16 cases underwent allogeneic orthotopic liver transplantation with improved two-cuff and hepatic artery reconstruction methods, which were divided into acute rejection and control group (8 cases for each group). Immunosuppressive therapy was not applied in the acute rejection group, but was applied in the control group during recipients' perioperative period. Liver tissue after transplantion were obtained respectively at 6, 12, 24, 72 hours points to treated with hematoxylin-eosin (HE) staining, and then the degree of rejection was determined. The expression level of HSP 70 in liver tissue was detected by western blotting. The expression of HSP 70 in liver tissue was detected by immunohistochemistry.RESULTS AND CONCLUSION: Histological changes of liver acute rejection in acute rejection group were more serious than that in control group. Banff grade level in acute rejection group was higher than that in control group (P < 0.05). The expression levels of HSP 70 in both groups of transplanted liver were increased by immunohistochemistry and western blotting detection from the beginning to 72 hours after transplantation. However, the level of HSP 70 in acute rejection group was significantly higher than that in control group (P < 0.05). It is indicated that the early acute rejection of rhesus monkeys at 72 hours after transplantation was significantly observed without immunosuppressive therapy. The expression level of HSP 70 presents an obvious rising trend with the incidence and progress of acute rejection. HSP 70 has a high prediction and diagnostic value on early acute rejection after liver transplantation.