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Cosmids carrying Aspergillus terreus DNA can integrate into Saccharomyces cerevisiae chromosome XII via recombination between yeast and foreign DNAs |
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| Authors: | E A Shoubochkina I I Fodor |
| Institution: | (1) Institute of Biochemistry and Physiology of Microorganisms, USSR Academy of Sciences, Puschino-on-the Oka, 142292 Moscow region, USSR;(2) Present address: Biological Institute of Leningrad University, Oranienbaumskoye sch. 2, Stary Peterhof, 198904 Leningrad, USSR |
| Abstract: | Summary A genome clonotheque consisting of 25- to 40-kb Sau3A1 fragments of Aspergillus terreus DNA was constructed in the episomal cosmid vector pES33 containing the yeastARG4 gene. From the 475 transformants of cir° yeast strain ESH-0, 23 stable Arg + transformants were independently selected. Genetic and Southern analysis of these stable transformants showed that 39% arose as a result of recombination between cloned A. terreus DNA sequences and yeast chromosome XII. The recombination events most likely occurred in the regions of homology within the rDNA clusters of A. terreus and Saccharomyces cerevisiae. |
| Keywords: | Aspergillus terreus clonotheque Saccharomyces cerevisiae Homologous integration 2 |
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