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Development and characterization of nine polymorphic microsatellite markers from the sword prawn Parapenaeopsis hardwickii using PCR-based isolation of microsatellite arrays (PIMA)
Authors:Chuan-Wen Ho  Tzong-Der Tzeng  Tzen-Yuh Chiang  Chih-Yao Li  Feng-Jiau Lin
Affiliation:1. Department of Oceanography, National Sun Yat-sen University, Kaohsiung 804, Taiwan
2. Department of Leisure, Recreation and Tourism Management, Shu-Te University, Kaohsiung 824, Taiwan
3. Department of Life Sciences, National Cheng Kung University, Tainan 701, Taiwan
Abstract:Nine polymorphic microsatellite makers were isolated and characterized in 20 individuals of Parapenaeopsis hardwickii collected from the fishing ground near Ilan, Taiwan. Number of alleles per locus ranged from 3 to 8, observed and expected heterozygosities ranged from 0.5263 to 0.7632, and from 0.4225 to 0.8044, respectively. Only one locus showed a Hardy–Weinberg equilibrium deviation. No linkage disequilibrium was found among these studied loci. This set of microsatellites provides a suitable tool for population genetic structure and fishery management of this species in Asian coastal waters.
Keywords:
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