中西药联合应用对呼吸道合胞病毒抑制作用的研究

目的 了解病毒唑、护肺口服液及二者联合应用时对呼吸道合胞病毒(RSV)的体外抑制作用。方法 观察不同药物浓度下RSV对Hep—2细胞的致病作用。结果 病毒唑完全抑制RSV复制的最小浓度为7．80μg／ml,护肺口服液则为5．00mg／ml;两药联合应用时,完全抑制RSV复制的病毒唑和护肺口服液的最小浓度分别为减少到0．98μg／ml和0．63mg／m1,比单独用药浓度均降低了87．4％。结论 两种药物对RSV都有体外抑制作用,联合应用时效果最强。

In vitro inhibition of respiratory syncytial virus with combined ribavirin and an oral preparation of traditional Chinese medicine Hu Fei

OBJECTIVE: To study the in vitro antiviral effect of ribavirin combined with an oral preparation of traditional Chinese medicine "Hu Fei" (protecting the lung) on respiratory syncytial virus (RSV). METHODS: Cytopathic effects (CEP) of RSV on Hep2 cells were observed after adding different concentrations of ribavirin, Hu Fei and combination of both into the culture medium. RESULTS: The minimum concentration of ribavirin and Hu Fei for complete inhibition of CPE caused by RSV was 7.80 microg/ml and 5.00 mg/ml, respectively. When the combination of ribavirin and Hu Fei was applied, their minimum concentrations needed for complete inhibition were decreased to 0.98 ?g/ml and 0.63 mg/ml. CONCLUSIONS: Both ribavirin and Hu Fei showed in vitro anti-RSV effect, but the inhibitory effect of combined ribavirin and Hu Fei was more potent than either of the preparation alone.