哮喘患者外周血单个核细胞CD4~+CD25~+调节性T细胞及IL-4分泌水平的检测

目的:探讨CD4+CD25+调节性T细胞在哮喘发病机制中的作用,为哮喘的临床治疗提供新的思路和方法。方法:分离哮喘患者和正常人外周血单个核细胞(PBMC),用流式细胞仪分析CD4+CD25+调节性T细胞在外周血单个核细胞中的比例;酶联免疫吸附法(ELASA)检测外周血PBMC培养上清IL-4的分泌状况。结果:哮喘组外周血CD4+CD25+调节性T细胞的百分率为(3.2±1.5)%,健康对照组为(6.5±2.5)%,哮喘组明显低于对照组(P<0.05);哮喘组外周血PBMC培养上清IL-4分泌水平为(57.3±13.5)ng/L,健康对照组为(28.6±7.2)ng/L,哮喘组明显高于对照组(P<0.05)。结论:CD4+CD25+调节性T细胞可能参与了哮喘的发生与发展。

Evaluation of CD4+CD25+ regulatory T cells and interleukin-4 levels in peripheral blood of asthma

Objective:To investigate the role of CD4+CD25+ regulatory T cells in the pathogenesis of asthma for the purpose of giving new approach of treatment.Methods:The monouclear cells were isolated from peripheral blood of asthmatic patients and control subjects,and the percentagn of CD4+CD25+ regulatory T cells was detemined by flow cytometry.The concentrations of IL-4 in PBMC supernatants were measured with enzyme linked immunosorbent assay (ELISA) method.Results:The percentage of CD4+CD25+ regulatory T cells in PBMC has significantly difference between control group(6.5±2.5)% and asthmatic patients (3.2±1.5)%(P<0.05).The concentrations of IL-4 in PBMC supernatants has significantly difference between control group(28.6±7.2) ng/L and asthmatic patients(57.3±13.5) ng/L(P<0.05).Conclusion:The CD4+CD25+ regulatory T cells involved in the pathogenesis of asthma in human.