两种干扰素抗Tca8113、ACC—M体外增殖的比较研究

目的比较干扰素α和干扰素γ分别对舌鳞状细胞癌(Tca8113)和腺样囊性癌(ACC-M)直接抗癌作用及抗癌特异性.方法采用MTT法和集落法(HTCA),分别检测两种干扰素对Tca8113、ACC-M及L929细胞增殖活性的影响.结果1000U/ml干扰素α、干扰素γ对Tca8113细胞生长抑制率分别为(45.4±4.1)%,(45.9±5.4)%;集落形成抑制率分别为(52.0±2.9)%,(54.6±4.1)%;对ACC-M细胞生长抑制率分别为(23.1±2.1)%,(26.5±1.3)%;集落形成抑制率分别为(26.2±2.0)%,(27.3±1.8)%.两种干扰素之间无显著性差异(P＞0.05),两种细胞系(株)之间有显著性差异(P＜0.05).1000U/ml干扰素α、干扰素γ对L929细胞生长抑制率分别为(3.9±1.1)%,(4.1±0.7)%,与Tca8113相比有显著性差异(P＜0.01).两种干扰素均有较好的抗癌作用特异性.结论干扰素α、干扰素γ对舌鳞状细胞癌有较强的直接抗癌作用和较好的抗癌特异性,但对腺样囊性癌抑制作用不明显.

Comparison of Anti-Proliferation Effects of Two IFNs on Tca8113,ACC-Min Vitro

OBJECTIVE: Comparison of anti-proliferation and anti tumor specificity of two IFNs on Tca8113, ACC-M in Vitro. METHODS: MTT assay and HTCA were performed to determine direct anti-proliferation effects of two IFNs on Tca8113, ACC-M and L929 cells, respectively. RESULTS: When Tca8113 cells were treated with 1000U/ml alpha-IFN, gamma-IFN, respectively, cell proliferation inhabition rate was (45.4+/-4.1)% by gamma-IFN, (45.9+/-5.4)% by gamma-IFN; inhibition rate of colony formation was (52.0+/-2.9)% by alpha-IFN, (54.6+/-4.1)% by gamma-IFN; and ACC-M cell proliferation inhibition rate was (23.1+/-2.1)% by alpha-IFN,(26.5+/-1.3)% by gamma-IFN; inhibition rate of colony formation was (23.1+/-2.1)% by alpha-IFN, (26.5+/-1.3)% by gamma-IFN; inhibition rate of colony formation was (26.2+/-2.0)% by alpha-IFN, (27.3+/-1.8)% by gamma-IFN. There was no significant difference between two IFNs (P>0.05). When L929 cells were treated with 1000U/ml alpha-IFN, gamma-IFN,respectively, cell proliferation inhibition rate was (3.9+/-1.1)% by alpha IFN, (4.1+/-0.7)% by gamma-IFN; There was significant difference in antiproliferation on Tca8113 cells and L929 cells (P<0.01). CONCLUSION: Both alpha-IFN and gamma-IFN have better direct anti-cancer effect on tongue squamous cell cancer and anti-tumor specificity. But anti-proliferation effect of two IFNs on ACC-M cells was not obvious.