低功率CO2激光焊接鼠小肠的机制初探

目的比较激光焊接与传统缝合肠管吻合口的胶原和DNA含量在愈合过程中的变化。方法将60只SD大鼠分为两组,每组30只,分别为缝合组和激光组,每组再分为3小组:第3天组(n=10)、第7天组(n=10)和第14天(n=10)。肌肉注射10%水合氯醛0.5 mg/Kg。腹部正中切口,每只SD大鼠均完成一个吻合口,距回盲部10 cm的回肠处切除回肠约1.5 cm。分别于术后第3、7和14天取吻合口近远端各0.5 cm的小肠组织采用比色法和二苯胺法分别测定其羟脯氨酸和DNA含量。结果吻合口组织羟脯氨酸含量:术后3 d缝合组为(0.87±0.16)μg/mg pro,激光组为(0.70±0.18)μg/mg pro,差异有显著意义(P<0.05);术后7 d激光组含量为(1.00±0.19)μg/mg pro,缝合组为(0.94±0.21)μg/mg pro,差异无显著意义(P>0.05)。术后14 d缝合组为(1.05±0.18)μg/mg pro,激光组为(1.32±0.11)μg/mg pro,差异有非常显著意义P<0.001。吻合口DNA含量:缝合第3天术后组为1.85±0.34 mg/g,激光组为(1.55±0.45)mg/g,差异有显著意义(P<0.05)。第7天缝合组为(1.95±0.43)μg/g,激光组为(1.75±0.36)mg/g,差异无显著意义(P>0.05)。第14天缝合组为(2.04±0.38)μg/g,激光组为(2.95±0.53)μg/g差异有非常显著意义(P<0.001)。结论激光焊接可以造成胶原变性,激光焊接还具有促进组织增生、细胞增殖、加速愈合的作用。

Intestinal Anastomosis with Low-power CO2 Laser in SD Rats: a PreliminaryResearch

Objective To compare the two different methods of collagen content and DNA in the healing process. Methods We divided 60 SD rats into two groups at random,suture group(n=30)and laser group(n=30).The animals were anesthetized with a solution of 10% chloral hydrate(0.5mg/kg) intramuscularly.A midline laparotomy was performed in all animals,we took out a segment of ileum which was 10cm farther from ilecaecum and 1.5cm ileum was resected for welding.Animals were sacrificed at 3,7and 14 days postoperatively and ...