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| ZnPcS4-光动力疗法对人视网膜色素上皮细胞的作用 | |
| 引用本文： | 黄焱,徐国兴,彭亦如. ZnPcS4-光动力疗法对人视网膜色素上皮细胞的作用[J]. 中国激光医学杂志, 2011, 20(6): 341-345,404 |
| 作者姓名： | 黄焱 徐国兴 彭亦如 |
| 作者单位： | 1. 福建医科大学医学技术与工程学院眼科学与视光学系 福州市,350004 2. 福建医科大学医学技术与工程学院眼科学与视光学系 福州市,350004 ;福建医科大学附属第一医院眼科 福州市,350004 3. 福建师范大学化学与材料学院 福州市,350004 |
| 基金项目： | 国家自然科学基金(20604007)福建省青年科技人才创新项目 |
| 摘 要： | 目的探讨四磺酸酞菁锌（ZnPcS4）介导的光动力疗法（PDT）对人视网膜色素上皮细胞的作用。方法从意外死亡者眼球中分离出视网膜色素上皮（RPE）细胞,用紫外可见光谱分析仪观察RPE细胞对ZnPcS4的摄取及代谢的规律,确定PDT的最佳时间点,根据激光能量密度分为50和100J／cm^2组。用MTT计算RPE细胞的抑制率。PDT后1h,用荧光显微镜及流式细胞仪观察和检测细胞内活性氧的含量。结果光照对RPE细胞的抑制率随激光能量密度增强而增强。ZnPcS。介导的PDT作用后,RPE细胞内活性氧含量PDT组与对照组比较,差异有非常显著意义。结论RPE细胞对ZnPcS~的吸收4h达到高峰。ZnPcS。介导的PDT作用后,RPE细胞内活性氧含量显著增加,对细胞有一定的损伤作用。 |
| 关 键 词： | 光动力疗法 视网膜色素上皮 活性氧 |
Photodynamic Effects of ZnPcS4 on Human Retinal Pigment Epithelium Cell |
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| HUANG Yan,XU Guo-xing,PENG Yi-ru. Photodynamic Effects of ZnPcS4 on Human Retinal Pigment Epithelium Cell[J]. Chinese Journal of Laser Medicine & Surgery, 2011, 20(6): 341-345,404 | |
| Authors: | HUANG Yan XU Guo-xing PENG Yi-ru |
| Affiliation: | HUANG Yan1,XU Guo-xing1,2,PENG Yi-ru3 1.Department of Ophthalmology and Optometry,Fujian Medical University,Fuzhou,Fujian 350004,China 2.Department of Ophthalmology,the First Affiliated Hospital of Fujian Medical University 3.College of Chemistry & Materials,Fujian Normal University |
| Abstract: | Objective To investigate the effects of phototoxicity on human retinal pigment epithelial(RPE) cells after zinc tetra-sulfonated phthalocyanine(ZnPcS4) based photodynamic therapy(PDT). Methods The 2-4 generations of subcultured RPE cells were used in the study.Firstly,the cellular uptake of ZnPcS4 was quantified by UV-spectra after confluent RPE cells were exposed to various doses of ZnPcS4 for 1-6 hrs.Secondly,RPE cells were divided into four groups: the control group,the group of photosensitizer without i... |
| Keywords: | Photodynamic therapy Retinal pigment epithelium Reactive oxygen species |
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