171株医院感染葡萄球菌的耐药性分析

目的了解我院2006—2007年导致医院感染的葡萄球菌分类及耐药性。方法采用常规方法对我院医院感染患者的各类标本进行分离培养，用生物梅里埃公司的VITEK-32细菌鉴定仪对分离的细菌进行鉴定，然后用纸片扩散法（K-B）进行药物敏感性试验，用WHONET5．3进行统计学分析。结果共分离出葡萄球菌171株，其中金黄色葡萄球菌48株（28．1％），凝固酶阴性葡萄球菌123株（71．9％）。耐甲氧西林的金黄色葡萄球菌（MRSA）检出率为31．3％；耐甲氧西林凝固酶阴性葡萄球菌检出率为37．4％。耐药率监测表明耐甲氧西林葡萄球菌（MRS）对10种常用抗菌药物耐药率远远高于甲氧西林敏感葡萄球菌（MSS）。结论应高度重视MRS的检测和报告，为临床提供可靠的诊断和治疗依据，及时有效的监测和控制葡萄球菌医院感染。

Antibiotics Resistance of 171 Staphylococcus strains in Nosocomial Infection

Objective To investigate the distribution and drug resistance of 171 Staphylococcus strains in nosocomial infection from 2006 to 2007 in our hospital. Methods Strains collected from daily specimens were identified with VITEK-32, and then the drug resistances were measured by standard K-B methods. The antimicrobial resistance rates were analyzed byWHONE15.3 software. Results Among 171 Staphylococcus strains collected, Staphylococcus aureus were predominant （28.1% ）, and the remains were all coagulase-negative Staphylococcus. Meticillin-resistant Staphylococcus aureus（MRSA） accounted for 31.3 %, and metieillin-resistant coagulase-negative Staphylococcus（MRCNS） accounted for 37.4%. The drug sensitivity results showed that metieillin-resistant Staphylococcus（MRS）was highly resistant to 10 commonly used antibiotics than meticillin-sensitive Staphylococcus（MSS）.Condusion For effective supervision and control of Staphylococcus infection, detection of MRS should be paid much attention by clinical microbiology laboratory.