枸杞叶、桑叶水提物体外抑菌活性研究

目的：研究枸杞叶、桑叶水提物对常见的病原微生物的体外抑菌作用。方法：将枸杞叶和桑叶提取物用试管二倍稀释法联合琼脂平板法，进行体外抑菌试验。结果：枸杞叶水提物对金黄色葡萄球菌最小抑菌浓度（ MIC ）为10 mg/mL，对表皮葡萄球菌MIC为20 mg/mL，对大肠杆菌、铜绿假单胞菌、白假丝酵母菌MIC均＆gt;20 mg/mL；桑叶水提取物对金黄色葡萄球菌的MIC为20 mg/mL；对表皮葡萄球菌、大肠杆菌、绿脓杆菌、白色念珠菌MIC均＆gt;20 mg/mL。结论：枸杞叶和桑叶水提物对金黄色葡萄球菌有抑菌作用，两者对革兰阴性菌、念珠菌抑菌作用不显著。

The Study of Bacteriostatic ActiVity of Aqueous EXtract from WoIfberry Leaf and MuIberry Leaf

ObjectiVes：To identify the antibacterial actiVity of aqueous eXtract from wolfberry leaf and mulberry leaf against common pathogenic microorganisms in Vitro. Methods：The antibacterial actiVity of aqueous eXtract from wolfberry leaf and mul-berry leaf was identified by AGAR plate method and tube double dilution method. ResuIts：The minimum bacteriostatic concen-tration（ MIC）of wolfberry leaf aqueous eXtract on Staphylococcus aureus and Epidermis staphylococcus was 10 mg/mL and 20 mg/mL respectiVely. The MIC of wolfberry leaf aqueous eXtract on Escherichia coli,Pseudomonas aeruginosa or White candi-da was greater than 20 mg/mL. Besides,the MIC of mulberry leaVes eXtract on Staphylococcus aureus was 20 mg/mL and The MIC on Epidermis staphylococcus,Escherichia coli,Pseudomonas aeruginosa or Candida albicans was greater than 20 mg/mL. ConcIusion：The minimum bacteriostatic concentration（ MIC）of wolfberry leaf eXtract on Staphylococcus aureus was 10 mg/mL, The minimum bacteriostatic concentration（ MIC）of mulberry leaf aqueous eXtract on Staphylococcus aureus was 20 mg/mL. The effect of both on gram-negatiVe bacteria,candida was not significant.