骨桥蛋白在卵巢上皮性癌组织中的表达及其意义

目的:探讨骨桥蛋白(OPN)在正常卵巢组织、卵巢良性肿瘤组织及卵巢上皮性癌组织中的表达情况,阐明OPN作为监测卵巢上皮性癌恶性程度和预后评估指标的价值。方法:收集卵巢切除标本共100例,其中正常卵巢组织切除标本20例,卵巢良性肿瘤组织切除标本20例,卵巢上皮性癌组织切除标本60例。卵巢上皮性癌包括卵巢浆液性囊腺癌30例、卵巢黏液性囊腺癌20例、卵巢子宫内膜样癌10例;按分化程度不同分为高分化癌(G1)12例、中分化癌(G2)16例、低分化癌(G3)32例(G1和G2组合并后与G3比较);按国际妇产科联盟(FIGO)2000年对原发性卵巢恶性肿瘤的手术-病理分期标准,Ⅰ、Ⅱ期卵巢上皮性癌组织切除标本30例,Ⅲ、Ⅳ期卵巢上皮性癌组织切除标本30例。采用HE染色和免疫组织化学染色检测OPN表达,分析OPN表达与卵巢上皮性癌临床病理特征的关系。结果:OPN阳性表达主要定位于癌细胞的细胞浆。OPN在60例卵巢上皮性癌组织中的阳性表达率(75.0%)高于正常卵巢组及卵巢良性肿瘤组(P<0.01)。随临床分期的增高,OPN阳性表达率均增高;低分化(G3)卵巢上皮性癌组织中OPN阳性表达率(93.8%)明显高于中高分化组(G1和G2)OPN阳性表达率(53.6%)(P<0.01)。不同组织学分型间OPN阳性表达率比较差异无统计学意义(P>0.05)。结论:OPN可能促进卵巢上皮性癌的发生、发展、侵袭及转移。OPN对于卵巢上皮性癌的临床诊断和转移评估具有重要意义,可作为监测卵巢上皮性癌恶性程度和预后评估的一个重要指标。

Expression of osteopontin in human epithelial ovarian carcinoma tissue and its significance

Objective To explore the expressions of osteopontin(OPN)in normal ovarian tissue,benign ovarian tumor tissue and epithelial ovarian carcinoma tissue,and to clarify the value of OPN as an indicator to monitor the malignancy degree and evaluate the prognosis of epithelial ovarian carcinoma.Methods The ovarian resection specimens of 100cases were collected,including 20cases of normal ovarian tissue,20cases of benign ovarian tumor tissue and 60cases of epithelial ovarian carcinoma tissue.Epithelial ovarian carcinoma including 30cases of ovarian serous adenocarcinoma,20 cases of ovarian mucinous cystadenocarcinoma and 10 cases of ovarian endometrioid carcinoma.According to the differentiation degree,60cases of epithlial ovarian carcinoma were divided into 12cases well differentiated carcinoma(G1),16cases of moderately differentiated carcinoma(G2),and 32cases of poorly differentiated carcinoma(G3).According to the International Federation of Gynecology and Obstetrics(FIGO)2000primary ovarian cancer surgery-pathological staging criteria,60cases of epithelial ovariancarcinoma including 30cases of epithelial ovarian carcinoma at stageⅠ and Ⅱ,and 30cases of epithelial ovarian carcinoma at stage Ⅲ and Ⅳ.HE staining and immunohistochemical staining were used to detect the OPN expression.Results OPN mainly expressed in the cytoplasm of cancer cells.The positive expression rate of OPN in 60cases of epithelial ovarian carcinoma group was 75.0% which was higher than those in normal ovarian group and benign ovarian tumor group(P<0.01).With the increasing of clinical stage,the positive expression rate of OPN was increased;the positive expression rate of OPN in poorly differentiated epithelial ovarian carcinoma group was higher than that in well differentiated epithelial ovarian carcinoma group(P<0.01);the positive expression rates of OPN had no significant differences between different histological types of epithelial ovarian carcinoma(P> 0.05).Conclusion OPN may contribute to the occurrence,development,invasion,and metastasis of epithelial ovarian carcinoma.OPN is of great significance for the clinical assessment of diagnosis and metastasis of epithelial ovarian carcinoma,and it can be used as an important indicator to monitor the malignancy degree and evaluate the prognosis of epithelial ovarian carcinoma.