| 多囊肾患者家系血清miRNA-15a和miRNA-17表达水平的比较 |
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| 引用本文： | 陈钦开,王满庭,杨柳,阮琼芳,杨阳,谢安,汪泱.多囊肾患者家系血清miRNA-15a和miRNA-17表达水平的比较[J].江西医学检验,2011(1):1-2,8. |
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| 作者姓名： | 陈钦开 王满庭 杨柳 阮琼芳 杨阳 谢安 汪泱 |
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| 作者单位： | 南昌大学第一附属医院肾内科;南昌大学研究生院医学部;南昌大学第一附属医院泌外研究所; |
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| 基金项目： | 国家自然科学基金(30960385); 江西省自然科学基金(2010GZY0333) |
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| 摘 要： | 目的观察比较miRNA-15a和miRNA-17在多囊肾患者家系中的表达水平,探讨miRNA与多囊肾病发生发展的可能关系。方法采用实时定量PCR技术检测多囊肾患者及其家系成员与正常人血清中miRNA-15a和miRNA-17的表达水平。结果所检测的多囊肾患者家系中已出现肾功能损害的多囊肾患者血清miRNA-15a的表达水平明显高于正常人及肾功能正常的多囊肾患者;而miRNA-17在多囊肾患者家系未表达。结论多囊肾患者血清miRNA-15a表达水平与正常人不同,提示miRNA在多囊肾的发生发展过程中可能起重要调控作用,值得深入研究。
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| 关 键 词： | miRNA 多囊肾病 实时定量PCR |
Comparison of serum miRNA-15a and miRNA-17 levels in patient families with polycystic kidney disease |
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| CHE Qinkai,WANG Manting,YANG Liu,et al..Comparison of serum miRNA-15a and miRNA-17 levels in patient families with polycystic kidney disease[J].Jiangxi Journal of Medical Laboratory Sciences,2011(1):1-2,8. |
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| Authors: | CHE Qinkai WANG Manting YANG Liu |
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| Institution: | CHE Qinkai,WANG Manting,YANG Liu,et al.Urology Institute,The First Affiliated Hospital of Nanchang University,Nanchang 330006,China |
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| Abstract: | Objective To explore the relationship between serum miRNA and the occurrence or development of polycystic kidney disease in patient families.Method Serum levels of miRNA-15a and miRNA-17 of patients with polycystic kidney disease including their family members and the controls were measured by real-time PCR.Results Compared with the controls,the cases of polycystic kidney disease with renal dysfunction showed a higher level expression of miRNA-15a,but miRNA-17 expression were not found in the patient group.... |
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| Keywords: | miRNA Polycystic kidney disease Real time PCR |
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