乙、丙型肝炎病毒重叠感染的慢性肝病患者肝组织和血清中的病毒检测

为了探讨乙型和丙型肝炎病毒（ＨＢＶ和ＨＣＶ）重叠感染者病毒相互干扰现象，对４４例患者血清检测诊断为ＨＢＶ和ＨＣＶ感染的慢性肝病患者，用原位杂交法检测了肝组织中ＨＢＶＤＮＡ和ＨＣＶＲＮＡ。２８例患者肝组织ＨＢＶＤＮＡ和ＨＣＶＲＮＡ同时阳性，两者阳性信号分布类型比较无明显差异（Ｐ＞０．０５），血清ＨＢｅＡｇ阳性和阴性组中肝组织中的ＨＢＶＤＮＡ和ＨＣＶＲＮＡ检出率无明显差异（Ｐ＞０．０５）。８例血清ＨＢｓＡｇ阴性者，肝组织ＨＢＶＤＮＡ阳性５例，ＨＣＶＲＮＡ阳性３例。结果提示，ＨＢＶ和ＨＣＶ重叠感染肝组织损伤加重，可能诱导一种病毒呈现潜伏状态，但不是病毒清除。

VIRUS DETECTION IN THE LIVER TISSUES AND SERUM FROM THE CASES OF CHRONIC LIVER DISEASE FOR THE SUPERINFECTON OF HEPATITIS B AND C VIRUSES

In order to investigate the interference phenomenon of different viruses in the patients with superinfection of hepatitis B and C viruses (HBV and HCV), HBV DNA and HCV RNA of the liver tissues were detected by situ ribonuclear probes in 44 patients who were diagnosed as superinfection of HBV and HCV by ELISA. 28 cases were found positive for both HBV DNA and HCV RNA in the liver tissues. There was no difference between distribution type of two positive signals in liver tissues (P>0.05). There was no difference in the detection rate of HBV DNA and HCV RNA between the HBeAg positive and negative groups (P>0.05). In 8 patients with HBsAg negative, 5 cases were positive for HBV DNA and 3 cases were positive for HCV RNA in liver tissues. The results suggested that the superinfection of HBV and HCV might lead aggravation of the liver disease and induce a virus into state of concealment, but was not eliminated.